Functional localization of single active ion channels can the surface of a living cell
Functional localization of single active ion channels can the surface of a living cell
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DOI：
10.1038/35023563
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发表时间：
2000-09-01
影响因子：
21.3
通讯作者：
Lab, MJ
中科院分区：
文献类型：
--
作者：
Korchev, YE;Negulyaev, YA;Lab, MJ
细胞质膜中离子通道的空间分布在控制区域特化方面具有重要作用，从而提供对细胞功能的精确和局部控制。我们在这里报告一种新的技术，扫描离子电导显微镜的基础上，允许，第一次，在完整的细胞质膜的单个活性离子通道的映射。我们绘制了心肌细胞ATP调节的K+通道（K-ATP通道）的分布。这些通道被组织成小组，并锚定在肌膜的Z槽中。这些通道的独特分布模式可能具有重要的功能意义。
The spatial distribution of ion channels in the cell plasma membrane has an important role in governing regional specialization, providing a precise and localized control over cell function. We report here a novel technique based on scanning ion conductance microscopy that allows, for the first time, mapping of single active ion channels in intact cell plasma membranes. We have mapped the distribution of ATP-regulated K+ channels (K-ATP channels) in cardiac myocytes. The channels are organized in small groups and anchored in the Z-grooves of the sarcolemma. The distinct pattern of distribution of these channels may have important functional implications.