The role of specific 2′-hydroxyl groups in the stabilization of the folded conformation of kink-turn RNA
The role of specific 2′-hydroxyl groups in the stabilization of the folded conformation of kink-turn RNA
复制标题
DOI：
10.1261/rna.285707
复制
发表时间：
2007-02-01
期刊：
影响因子：
4.5
通讯作者：
Lilley, David M. J.
中科院分区：
文献类型：
--
作者：
Liu, Jia;Lilley, David M. J.
研究了 2'-羟基在稳定扭结转角（K 转角）紧密扭结几何形状方面的作用。通过化学合成去除了各个 2'-OH 基团，并通过凝胶电泳和荧光共振能量转移研究了 RNA 的扭结。参考 11 种不同 K 转晶体结构的数据库对结果进行了分析。潜在的氢键分为几类。最重要的是转角核心的那些以及凸起周围的核糖-磷酸相互作用。其中最重要的一个氢键是从凸起的 59 个核苷酸的 2'-OH 到扭结近端 A 中心点 G 对的腺嘌呤的 N1 的氢键。这存在于所有已知的 K 转结构中，并且去除 2'-OH 可以完全防止金属离子诱导的折叠。螺旋茎小凹槽中形成的氢键不太重要，去除参与的 2'-OH 基团可减少折叠损伤。这些相互作用通常更具多态性，并且在整体结构允许的情况下可能会形成氢键。
The role of 2'-hydroxyl groups in stabilizing the tightly kinked geometry of the kink-turn (K-turn) has been investigated. Individual 2'-OH groups have been removed by chemical synthesis, and the kinking of the RNA has been studied by gel electrophoresis and fluorescence resonance energy transfer. The results have been analyzed by reference to a database of 11 different crystallographic structures of K-turns. The potential hydrogen bonds fall into several classes. The most important are those in the core of the turn and ribose-phosphate interactions around the bulge. Of these the single most important hydrogen bond is one donated from the 2'-OH of the 59 nucleotide of the bulge to the N1 of the adenine of the kink-proximal A center dot G pair. This is present in all known K-turn structures, and removal of the 2'-OH completely prevents metal ion-induced folding. Hydrogen bonds formed in the minor grooves of the helical stems are less important, and removal of the participating 2'-OH groups leads to reduced impairment of folding. These interactions are generally more polymorphic, and hydrogen bonds probably form where possible, as permitted by the global structure.