Validity testing of the EZ-SCREEN cannabinoid test.
Validity testing of the EZ-SCREEN cannabinoid test.
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EZ-SCREEN 大麻素测试的有效性测试。
DOI：
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发表时间：
1993
影响因子：
2.5
通讯作者：
J. Mitchell
中科院分区：
文献类型：
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作者：
A. Jenkins;L. C. Mills;W. Darwin;M. Huestis;E. Cone;J. Mitchell
最近，一些“快速测试”可用于现场药物测试。这些测试具有简单、易于执行和快速访问测试结果的优点。然而，关于这些检验在发现药物滥用方面的有效性的数据很少。本报告描述了EZ-SCREEN大麻素检测尿液中大麻素的有效性研究。三名有大麻使用史的健康男性志愿者参加了这项研究。每个受试者在每个测试日吸1、2或4支大麻香烟（2.6%THC）。采集尿液样本，并将其纳入由178份临床尿液样本、72份含已知药物量的尿液样本和50份无药物尿液样本组成的样本集中。对样本集进行随机化，并在盲态条件下通过EZ-SCREEN试验和GC/MS分析11-去甲-9-羧基-Δ 9-四氢大麻酚（THCCOOH）。结果由三名阅片人独立解读。通过比较EZ-SCREEN试验与GC/MS的结果进行一致性分析。EZ-SCREEN试验具有高度灵敏度，并在5 ng/mL的标准THCCOOH浓度下产生阳性结果。虽然显示出对THCCOOH的高灵敏度，但该测定显示出与δ 9-四氢大麻酚（THC）和其他大麻素的低交叉反应性。50份不含药物的尿液样本没有记录假阳性结果，但一名读片员记录了8个未定结果。三位阅片师对EZ-Screen结果的总体一致性约为80%。延迟读数和影印读数往往不如3分钟时获得的读数准确。（250字处删节）
Recently, a number of "quick tests" became available for use in on-site drug testing. These tests offer advantages in simplicity, ease of performance, and rapid access to test results. However, there is a paucity of data on the validity of these tests for the detection of drugs of abuse. This report describes a validity study of the EZ-SCREEN cannabinoid test for the detection of cannabinoids in urine. Three healthy, male volunteers with a history of marijuana use participated in the study. Each subject smoked 1, 2, or 4 marijuana cigarettes (2.6% THC) on each test day. Urine samples were collected and incorporated into a specimen set consisting of 178 clinical urine samples, 72 urine samples containing known amounts of drug, and 50 drug-free urine samples. The specimen set was randomized and analyzed under blind conditions by the EZ-SCREEN test and by GC/MS for 11-nor-9-carboxy-delta 9-tetrahydrocannabinol (THCCOOH). Results were interpreted independently by three readers. Concordance analysis was performed by comparison of results of the EZ-SCREEN test with GC/MS. The EZ-SCREEN test was highly sensitive and produced positive results at a standard THCCOOH concentration of 5 ng/mL. While showing high sensitivity to THCCOOH, the assay demonstrated low cross-reactivity with delta 9-tetrahydrocannabinol (THC) and other cannabinoids. No false-positive results were recorded with 50 drug-free urine samples, but one reader recorded eight undecided results. Overall agreement between the three readers for the EZ-Screen results was approximately 80%. Delayed readings and photocopy readings tended to be less accurate than readings obtained at 3 min.(ABSTRACT TRUNCATED AT 250 WORDS)