Long-term dysfunction following diffuse traumatic brain injury in the immature rat
Long-term dysfunction following diffuse traumatic brain injury in the immature rat
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DOI：
10.1089/neu.2000.17.273
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发表时间：
2000-04-01
影响因子：
4.2
通讯作者：
Kochanek, PM
中科院分区：
文献类型：
--
作者：
Adelson, PD;Dixon, CE;Kochanek, PM
儿童严重弥漫性创伤性脑损伤（TBI）后常出现持续性认知功能障碍。为了研究弥漫性损伤对未成熟脑的影响，我们建立了未成熟大鼠严重弥漫性撞击（DI）加速TBI模型，并描述了创伤后早期运动和认知功能障碍。在本研究中，我们调查了长期功能能力后DI（150克/2米）相比，假在未成熟的（PND 17）大鼠。伤后10天内每天测量平衡木和倾斜平面倾斜度，以评估大体前庭功能。在DI和假损伤后3个月内每月评估Morris水迷宫（MWM）范式。DI大鼠在伤后24 h观察到平衡木和斜面上的乳酸减少（p < 0.05 vs. sham [n = 10/组]），并持续10 d，DI产生持续的MWM性能缺陷（p < 0.05 vs. sham），如在伤后90 d发现隐藏平台的更大乳酸显着所示。3个月后，损伤动物的脑重量和体重低于假手术组（p < 0.05）。我们得出结论，未成熟大鼠的弥漫性TBI：（a）在损伤后10天内产生了一致的，显著的，但可逆的运动缺陷;（B）在创伤后3个月内产生了长期的，持续的MWM性能缺陷;（c）在损伤后3个月内影响发育中大鼠的身体和大脑重量增加。这种TBI模型应该是有用的测试新的治疗方法和它们对未成熟大鼠的长期结果和发展的影响。
Children often suffer sustained cognitive dysfunction after severe diffuse traumatic brain injury (TBI), To study the effects of diffuse injury in the immature brain, we developed a model of severe diffuse impact (DI) acceleration TBI in immature rats and previously described the early motor and cognitive dysfunction posttrauma. In the present study, we investigated the long-term functional ability after DI (150 gm/2 m) compared to sham in the immature (PND 17) rat. Beam balance and inclined plane latencies were measured daily for 10 days after injury to assess gross vestibulomotor function. The Morris water maze (MWM) paradigm was evaluated monthly up to 3 months after DI and sham injuries. Reduced latencies on the balance beam and inclined plane were observed in DI rats (p < 0.05 vs. sham [n = 10 per group]) at 24 h and persisted for 10 days postinjury, DI produced sustained MWM performance deficits (p < 0.05 vs, sham) as indicated by the greater latencies to find the hidden platform remarkably through 90 days after injury. Lastly, the brain and body weights of the injured animals were less than sham (p < 0.05) after 3 months. We conclude that a diffuse TBI in the immature rat: (a) created a consistent, marked, but reversible motor deficit up to 10 days following injury; (b) produced a long-term, sustained performance deficit in the MWM up to 3 months posttrauma; and (c) affected body and brain weight gain in the developing rat through 3 months after injury. This TBI model should be useful for the testing of novel therapies and their effect on long-term outcome and development in the immature rat.