Serotyping of Toxoplasma gondii infections in humans using synthetic peptides
Serotyping of Toxoplasma gondii infections in humans using synthetic peptides
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DOI：
10.1086/374647
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发表时间：
2003-05-01
影响因子：
6.4
通讯作者：
Boothroyd, JC
中科院分区：
文献类型：
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作者：
Kong, JT;Grigg, ME;Boothroyd, JC
为了确定是否由于弓形虫（弓形虫病）的疾病的特点是依赖于感染菌株，我们已经开发了一种酶联免疫吸附试验分型菌株，使用感染血清对来自弓形虫抗原SAG 2A，GRA 3，GRA 6和GRA 7的多态性肽反应。对感染小鼠的初步研究确立了该方法的有效性，然后用人血清进行了测试。在Sabin-Feldman染料试验滴度>64并且感染菌株类型已知的8名患者中，肽正确区分了II型和非II型感染。对第二组10名未分离出寄生虫株的感染孕妇进行ELISA分析，明确预测了引起感染的菌株类型。这种方法应该允许获得关于不同菌株类型是否导致具有不同特征的疾病的统计学显著数据。
To determine whether the characteristics of disease due to Toxoplasma gondii (toxoplasmosis) are dependent on the infecting strain, we have developed an enzyme-linked immunosorbent assay for typing strains that uses infection serum reacted against polymorphic peptides derived from Toxoplasma antigens SAG2A, GRA3, GRA6, and GRA7. Pilot studies with infected mice established the validity of the approach, which was then tested with human serum. In 8 patients who had Sabin-Feldman dye test titers >64 and for whom the infecting strain type was known, the peptides correctly distinguished type II from non-type II infections. ELISA analysis of a second group of 10 infected pregnant women from whom the parasite strain had not been isolated gave a clear prediction of the strain type causing infection. This method should allow statistically significant data to be obtained about whether different strain types cause disease with different characteristics.