Intimate association of Thy-1 and the T-cell antigen receptor with the CD45 tyrosine phosphatase.
Intimate association of Thy-1 and the T-cell antigen receptor with the CD45 tyrosine phosphatase.
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Thy-1 和 T 细胞抗原受体与 CD45 酪氨酸磷酸酶密切相关。
DOI：
10.1073/pnas.87.18.7085
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发表时间：
1990
影响因子：
11.1
通讯作者：
J. Ashwell
中科院分区：
文献类型：
--
作者：
S. Volarevic;C. Burns;J. Sussman;J. Ashwell
免疫沉淀的Thy-1从Triton X-100洗涤剂裂解物的表面碘化和化学交联的T细胞沉淀至少五个主要的和离散的带。其中4条被鉴定为Thy-1、CD 45（一种跨膜酪氨酸磷酸酶）、一种主要组织相容性复合物编码的I类分子和β 2-微球蛋白。类似的分析表明，CD 45是由T细胞抗原受体（TCR）的抗体从交联剂处理的细胞的裂解物共沉淀。当毛地黄皂苷用于裂解未处理的细胞时，观察到相同的共沉淀条带模式。免疫沉淀的Thy-1或TCR从交联的T细胞的裂解物沉淀CD 45酪氨酸磷酸酶活性。基于共沉淀的酶活性或TCR ζ链的量的计算表明，Thy-1和TCR复合物的大部分可以与CD 45交联。这些数据支持一个模型，其中的依赖性的Thy-1信号对TCR共表达是由于它们共同的相互作用与酪氨酸磷酸酶，并提供了一个可能的结构基础的影响CD 45对TCR介导的信号。
Immunoprecipitation of Thy-1 from Triton X-100 detergent lysates of surface-iodinated and chemically cross-linked T cells precipitated at least five major and discrete bands. Four of these bands were identified as Thy-1, CD45 (a transmembrane tyrosine phosphatase), a major histocompatibility complex-encoded class I molecule, and beta 2-microglobulin. Similar analyses revealed that CD45 was coprecipitated from lysates of cross-linker-treated cells by antibodies to the T-cell antigen receptor (TCR). The same pattern of coprecipitated bands was observed when digitonin was used to lyse untreated cells. Immunoprecipitation of Thy-1 or the TCR from lysates of cross-linked T cells precipitated CD45 tyrosine phosphatase activity. Calculations based upon the amounts of coprecipitated enzymatic activity or TCR zeta chain indicate that a substantial fraction of Thy-1 and TCR complexes can be cross-linked to CD45. These data support a model in which the dependence of Thy-1 signaling on TCR coexpression is due to their common interaction with a tyrosine phosphatase and provide a possible structural basis for the influence of CD45 on TCR-mediated signaling.