DNA and Mini-DNA barcoding for the identification of Porgies species (family Sparidae) of commercial interest on the international market
DNA and Mini-DNA barcoding for the identification of Porgies species (family Sparidae) of commercial interest on the international market
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DOI：
10.1016/j.foodcont.2014.09.025
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发表时间：
2015-04-01
期刊：
影响因子：
6
通讯作者：
Guidi, Alessandra
中科院分区：
文献类型：
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作者：
Armani, Andrea;Guardone, Lisa;Guidi, Alessandra
鲷科物种的形态相似，其特点是不同的市场价格，代表了一个严重的问题，他们的贸易和库存管理，因为它鼓励欺诈替代。最受认可的形态学鉴定方法是基于牙板，但这种方法并不简单，不能用于制备产品。当使用分子方法时，烹饪引起的DNA降解是主要缺点。在这项工作中，我们收集了属于75个鲷科物种的314个参考组织，并从使用通用引物从新鲜和乙醇保存的组织中提取的DNA开始产生全（FDB）和迷你条形码（MDB）参考序列的数据集。此外，还烹饪了一些新鲜样品。FDB在91%（新鲜），50%（煮熟）和81%（乙醇保存）的样品中成功扩增，而MDB的扩增率在煮熟（100%）和乙醇保存（94%）的样品中相当高。使用相同的引物扩增从58个市售样品（MS）中获得的DNA。将所有DNA条形码与BOLD和GenBank进行ID和BLAST分析。FDB能够提供明确的物种水平的鉴定53（78%）和44（64.7%）的参考样品上分析的BOLD和GenBank，分别。微型DNA条形码（MDB）显示出较低的区分能力，32（45.7%）和29（41.4%）的序列与BOLD和GenBank上的物种明确匹配。然而，MDB允许将所有参考序列鉴定为属于鲷科。FDB和MDB在分析MS时表现出类似的性能，可以突出21个（38%）错误标记的MS。我们的研究在确认FOB作为鱼类认证的可靠工具的同时，提出了MOB作为一种有前途的工具来恢复熟产品的分子信息。(C)2014爱思唯尔有限公司版权所有。
The morphological similarity among Sparidae species, which are characterized by a different market price, represents a serious problem for their trade and for stock management, since it encourages frauds for substitution. The most accredited morphological method for their identification is based on the dental-plate, but this approach is not simple and cannot be used for prepared products. When molecular methods are used the DNA degradation induced by cooking is the main drawback. In this work, we collected 314 reference tissues belonging to 75 Sparidae species and we produced a dataset of full (FDB) and mini-barcode (MDB) reference sequences starting from DNA extracted from fresh and ethanol-preserved tissues using universal primes. Moreover, some fresh samples were cooked. The FDB was successfully amplified in 91% (fresh), 50% (cooked) and 81% (ethanol-preserved) samples, while the amplification rates of the MDB were considerably higher in case of cooked (100%) and ethanol-preserved (94%) samples. The same primers were used for the amplification of the DNA obtained from 58 market samples (MS). All the DNA barcodes were compared with BOLD and GenBank using IDs and BLAST analysis. FDB was able to provide unambiguous species-level identifications for 53 (78%) and 44 (64.7%) reference samples analyzed on BOLD and GenBank, respectively. The Mini-DNA barcode (MDB) showed a lower discriminating power with 32 (45.7%) and 29 (41.4%) sequences unambiguously matched to a species on BOLD and GenBank. However, the MDB allowed to identify all the reference sequences as belonging to the Sparidae family. FDB and MDB showed a similar performance in analyzing the MS, allowing to highlight 21(38%) mislabeled MS. Our study, while confirming the FOB as a reliable tool for fish authentication, proposes the MOB as a promising tool to recover molecular information in case of cooked products. (C) 2014 Elsevier Ltd. All rights reserved.