High temperature enhances anthocyanin coloration in Asiatic hybrid lily flowers via upregulation of the MYB12 positive regulator
High temperature enhances anthocyanin coloration in Asiatic hybrid lily flowers via upregulation of the MYB12 positive regulator
复制标题
DOI：
10.1016/j.hpj.2022.05.003
复制
发表时间：
2022-05
影响因子：
5.7
通讯作者：
M. Yamagishi
中科院分区：
文献类型：
--
作者：
M. Yamagishi
虽然高大气温度抑制花色素苷的积累，在大多数植物物种，我们表明，高温加速花色素苷色素沉着的亚洲杂交百合（百合属）的花被片。高温（35 °C）培养2 d后，两个亚洲百合品种的花被片大部分花色苷颜色变深，但“蒙特勒”花被片基部和“多伦多”花被片外层顶部变白。环境刺激常常影响R2 R3-MYB正调控因子的表达，而R2 R3-MYB正调控因子控制花青素苷的生物合成。虽然在其他物种中它们的表达经常被高温抑制，但在花被片中lilyMYB 12的表达被高温上调。MicroRNA 828（miR 828）在转录后抑制MYB 12的作用，在高温下表现出降低的积累水平，表明miR 828调节参与MYB 12上调。此外，通过非剪接MYB 12转录本积累估计的MYB 12转录水平也被高温激活。因此，miR 828的积累受到抑制，MYB 12的转录增加，这两种情况可能都参与了高温下MYB 12的激活。在35 °C下，“蒙特勒”花被片基部白化，bHLH 2的表达受到严重抑制，而MYB 12的表达不受影响。目前的研究结果表明，植物表现出不同的反应，热气候条件下，在不同的环境条件下花色素苷的调控提供了新的思路。
Although high atmospheric temperatures suppress anthocyanin accumulation in most plant species, we show that high temperatures accelerate anthocyanin pigmentation in flower tepals of Asiatic hybrid lilies (Liliumspp.). After incubation at high temperatures (35 °C) for two days, anthocyanin color became deeper in the most of tepal parts of two Asiatic hybrid lily cultivars, although the basal parts of ‘Montreux’ tepals and top parts of 'Toronto' outer tepals were whitened. Environmental stimuli often affect the expression ofR2R3-MYBpositive regulators that control anthocyanin biosynthesis. Although their expression is often suppressed by hot temperatures in other species, the expression of lilyMYB12in tepals was upregulated by high temperatures. MicroRNA828 (miR828), which suppresses the action ofMYB12post-transcriptionally, exhibited reduced accumulation levels under high temperature, indicating that miR828 regulation is involved inMYB12upregulation. In addition, transcription levels ofMYB12, estimated by unsplicedMYB12transcript accumulation, were also activated by high temperatures. Thus, both suppressed miR828 accumulation and increasedMYB12transcription are likely to be involved inMYB12activation at high temperatures. In the whitened basal parts of ‘Montreux’ tepals at 35 °C, expression ofbHLH2was severely suppressed while that ofMYB12was not affected. The present results demonstrate that plants display diverse responses to hot climatic conditions and shed new light on anthocyanin regulation under various environmental conditions.