Live-cell imaging of invasion and intravasation in an artificial microvessel platform.
Live-cell imaging of invasion and intravasation in an artificial microvessel platform.
复制标题
DOI：
10.1158/0008-5472.can-14-1042
复制
发表时间：
2014-09-01
期刊：
影响因子：
11.2
通讯作者：
Searson PC
中科院分区：
文献类型：
--
作者：
Wong AD;Searson PC
存在可视化转移的方法，但仍然需要额外的工具来更好地定义侵袭和内渗的生物和物理过程。研究转移的困难之一源于肿瘤微环境与血管系统之间界面的复杂性。在这里，我们报告了一个研究平台的开发，该平台将肿瘤细胞定位在嵌入细胞外基质（ECM）的人造血管旁边。在这个平台上，我们使用活细胞荧光显微镜来分析转移性癌细胞和内皮细胞排列的功能性人造微血管之间复杂的相互作用。该平台概括了已知的相互作用，其使用证明了系统研究涉及入侵和内渗的新物理和生物参数的能力。总之，我们的工作提供了一个重要的新工具来增进有关转移和候选抗转移疗法的知识。
Methods to visualize metastasis exist, but additional tools to better define the biological and physical processes underlying invasion and intravasation are still needed. One difficulty in studying metastasis stems from the complexity of the interface between the tumor microenvironment and the vascular system. Here we report the development of an investigational platform that positions tumor cells next to an artificial vessel embedded in an extracellular matrix (ECM). On this platform, we used live-cell fluorescence microscopy to analyze the complex interplay between metastatic cancer cells and a functional artificial microvessel which was lined with endothelial cells. The platform recapitulated known interactions, and its use demonstrated the capabilities for a systematic study of novel physical and biological parameters involved in invasion and intravasation. In summary, our work offers an important new tool to advance knowledge about metastasis and candidate anti-metastatic therapies.