Messenger RNA prevalence in sea urchin embryos measured with cloned cDNAs.
Messenger RNA prevalence in sea urchin embryos measured with cloned cDNAs.
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用克隆的 cDNA 测量海胆胚胎中信使 RNA 的流行率。
DOI：
10.1073/pnas.77.9.5317
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发表时间：
1980
影响因子：
11.1
通讯作者：
Davidson,EH
中科院分区：
文献类型：
--
作者：
Lasky,LA;Lev,Z;Xin,JH;Britten,RJ;Davidson,EH
用从未受精卵和胚胎转录的多聚(A)-RNA中转录的标记cDNA处理cDNA克隆克隆，以测量海胆发育期间的mRNA流行率。通过溶液中的Poly(A)-RNA滴定，独立地测定了与多个克隆互补的每个胚胎的细胞质转录子数量，在菌落杂交中，与这些克隆结合的cDNA量与各自胚胎细胞质中Poly(A)-RNAs的浓度成正比。在原肠胚期，最普遍的信使核糖核酸出现在每个胚胎大约10(6)个分子中。如果所有细胞都是等同的，那么每个细胞就有几百个分子。到了插管期，一些序列的患病率增加了10倍以上。在较晚的胚胎中普遍存在的大多数序列也存在于未受精卵的母体RNA中。对于大多数Poly(A)-RNA序列，在卵子发生过程中确定的流行水平在整个排卵期都保持不变，而少数序列在发育过程中表现出明显的阶段特异性表达变化。
mRNA prevalence during sea urchin development was measured by treating cDNA clone colonies with labeled cDNAs transcribed from unfertilized egg and embryo poly(A)-RNAs. The number of cytoplasmic transcripts per embryo complementary to several clones was determined independently by titration with poly(A)-RNA in solution, and the amount of cDNA bound to these clones in colony hybridizations was shown to be proportional to the concentration of the respective poly(A)-RNAs in the embryo cytoplasm. At the gastrula stage, the most prevalent mRNA species occur in about 10(6) molecules per embryo. If all cells were equivalent, this would be a few hundred molecules per cell. By pluteus stage, the prevalence of some sequences has increased more than 10-fold. Most, though not all, sequences prevalent in later embryos are also present in the maternal RNA of the unfertilized egg. For most poly(A)-RNA sequences, the prevalence levels determined during oogenesis are maintained through the pluteus stage, whereas a minority of sequences display sharp stage-specific changes in representation during development.