An Mψ-containing heterologous RNA, but not env mRNA, is efficiently packaged into avian retroviral particles
An Mψ-containing heterologous RNA, but not env mRNA, is efficiently packaged into avian retroviral particles
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DOI：
10.1128/jvi.73.11.8926-8933.1999
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发表时间：
1999-11-01
影响因子：
5.4
通讯作者：
Linial, ML
中科院分区：
文献类型：
--
作者：
Banks, JD;Kealoha, BO;Linial, ML
逆转录病毒优先包装全长基因组RNA，而不是拼接的病毒消息。对于大多数逆转录病毒来说，这种偏好可能是由于亚基因组RNA上缺乏全部或部分包装信号。然而，在禽白血病肉瘤病毒中，我们已经证明最小包装信号M Psi位于5‘剪接点的上游，因此存在于基因组和剪接RNA上。我们现在证明，含有M Psi的异源RNA的包装效率仅为基因组Rous肉瘤病毒RNA的2.6倍。因此，在M Psi之外几乎不存在附加的包装序列和/或结构。相反，我们发现env mRNA没有被有效包装，这些结果表明要么M Psi对这个RNA不起作用，要么RNA以某种方式从包装机械中分离出来。最后，M Psi 3‘端序列的缺失降低了异源RNA的包装效率。
Retroviruses preferentially package full-length genomic RNA over spliced viral messages. For most retroviruses, this preference is likely due to the absence of all or part of the packaging signal on subgenomic RNAs. In avian leukosis-sarcoma virus, however, we have shown that the minimal packaging signal, M Psi, is located upstream of the 5' splice site and therefore is present on both genomic and spliced RNAs, We now show that an M Psi-containing heterologous RNA is packaged only 2.6-fold less efficiently than genomic Rous sarcoma virus RNA. Thus, few additional packaging sequences and/or structures exist outside of M Psi. In contrast, we found that env mRNA is not efficiently packaged, These results indicate that either M Psi is not functional on this RNA or the RNA is somehow segregated from the packaging machinery. Finally, deletion of sequences from the 3' end of M Psi was found to reduce the packaging efficiency of heterologous RNAs.