RECA PROTEIN OF ESCHERICHIA-COLI PROMOTES BRANCH MIGRATION, A KINETICALLY DISTINCT PHASE OF DNA STRAND EXCHANGE
RECA PROTEIN OF ESCHERICHIA-COLI PROMOTES BRANCH MIGRATION, A KINETICALLY DISTINCT PHASE OF DNA STRAND EXCHANGE
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DOI：
10.1073/pnas.78.6.3433
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
LEHMAN, IR
中科院分区：
文献类型：
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作者：
COX, MM;LEHMAN, IR
重组E.大肠杆菌的DNA促进噬菌体v φ X-174的全长线性双链体和单链环状DNA分子之间的链的完全交换，将> 50%的单链DNA转化为异源双链体复制型II样结构。在动力学上，该反应可分为2个阶段，短异源双链体区域（D环）的形成和D环通过分支迁移的延伸。recA蛋白直接参与这两个阶段。D环在ATP或不可水解的ATP类似物腺苷5“-[γ-腺苷]存在下有效地形成。硫代]三磷酸; D环延伸需要连续的ATP水解。完整的链交换需要化学计量量的recA蛋白，并受到大肠杆菌单链DNA结合蛋白的强烈刺激。杆菌
The recA protein of E. coli promotes the complete exchange of strands between full-length linear duplex and single-stranded circular DNA molecules of bacteriophage .vphi.X-174, converting > 50% of the single-stranded DNA into heteroduplex replicative form II-like structures. Kinetically, the reaction can be divided into 2 phases, formation of short heteroduplex regions (D loops) and extension of the D loops via branch migration. recA protein participates directly in both phases. D loops are formed efficiently in the presence of ATP or the non-hydrolyzable ATP analog adenosine 5''-[.gamma.-thio]triphosphate; D-loop extension requires continuous ATP hydrolysis. Complete strand exchange requires a stoichiometric amount of recA protein and is strongly stimulated by the single-stranded-DNA-binding protein of E. coli.