Infection by retroviral vectors outside of their host range in the presence of replication-defective adenovirus.
Infection by retroviral vectors outside of their host range in the presence of replication-defective adenovirus.
复制标题
在复制缺陷型腺病毒存在的情况下，在宿主范围之外被逆转录病毒载体感染。
DOI：
10.1128/jvi.69.3.1887-1894.1995
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Ledley,FD
中科院分区：
文献类型：
--
作者：
Adams,RM;Wang,M;Steffen,D;Ledley,FD
逆转录病毒感染通常限于特定宿主范围内的细胞，其表达由env基因的产物识别的同源受体。我们描述了逆转录病毒感染的细胞以外的正常宿主范围时，进行感染的复制缺陷型腺病毒（dl 312）的存在下。在腺病毒存在下，几种不同的亲嗜性载体显示感染人细胞系（HeLa和PLC/PRF），并且异嗜性载体显示感染鼠细胞（NIH 3 T3）。通过5-溴-4-氯-3-吲哚基-β-D-吡喃半乳糖苷（X-Gal）染色、用G418选择新霉素抗性和PCR鉴定感染细胞中的前病毒来证明抗性。免疫力在数量上取决于腺病毒的浓度（10（6）至10（8）PFU/ml）和逆转录病毒的浓度。感染需要逆转录病毒感染培养基中同时存在腺病毒，并且在逆转录病毒感染前不通过预孵育和从细胞中去除腺病毒来刺激。腺病毒的存在显示出增强荧光标记的逆转录病毒颗粒摄取到其正常宿主范围之外的细胞中，表明腺病毒在不存在识别的同源受体的情况下增强病毒进入细胞。这一观察结果为开发用于基因治疗的安全逆转录病毒载体和逆转录病毒疾病发病机制提供了新的机会。
Retrovirus infection is normally limited to cells within a specific host range which express a cognate receptor that is recognized by the product of the env gene. We describe retrovirus infection of cells outside of their normal host range when the infection is performed in the presence of a replication-defective adenovirus (dl312). In the presence of adenovirus, several different ecotropic vectors are shown to infect human cell lines (HeLa and PLC/PRF), and a xenotropic vector is shown to infect murine cells (NIH 3T3). Infectivity is demonstrated by 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) staining, selection with G418 for neomycin resistance, and PCR identification of the provirus in infected cells. Infectivity is quantitatively dependent upon both the concentration of adenovirus (10(6) to 10(8) PFU/ml) and the concentration of retrovirus. Infection requires the simultaneous presence of adenovirus in the retrovirus infection medium and is not stimulated by preincubation and removal of adenovirus from the cells before retrovirus infection. The presence of adenovirus is shown to enhance the uptake of fluorescently labeled retrovirus particles into cells outside of their normal host range, demonstrating that the adenovirus enhances viral entry into cells in the absence of the recognized cognate receptor. This observation suggests new opportunities for developing safe retroviral vectors for gene therapy and new mechanisms for the pathogenesis of retroviral disease.