Measurement of blood flow through the retinal circulation of the cat during normoxia and hypoxemia using fluorescent microspheres
Measurement of blood flow through the retinal circulation of the cat during normoxia and hypoxemia using fluorescent microspheres
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DOI：
10.1006/mvre.2001.2321
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发表时间：
2001-09-01
影响因子：
3.1
通讯作者：
Linsenmeier, RA
中科院分区：
文献类型：
--
作者：
Ahmed, J;Pulfer, MK;Linsenmeier, RA
The most successful method for measuring absolute blood flow rate through the retinal circulation has been the use of radioactive microspheres. The purpose of this study was to develop a microsphere method that did not have the drawbacks associated with radioactivity and to use this method to make measurements of retinal blood flow in the cat. Blood flow measurements were made by injecting 15-mum-diameter polystyrene microspheres into the left ventricle of anesthetized, artificially ventilated cats. These microspheres were labeled with one of three fluorescent dyes. Retinal blood flow measurements were made by determining the number of spheres that were embedded in the retina and comparing them to the number found in a reference sample. Spheres in the retina were counted by making retinal whole mounts and taking retinal images with a CCD camera mounted on an epifluorescence microscope equipped with filter sets appropriate for imaging the dyes used to label the spheres. Blood flow measurements made under normal conditions showed a mean retinal blood flow of 19.8 +/- 12.4 ml/min 100 g tissue (mean +/- SD; n=15 cats). Since the retinal circulation perfuses only the inner half of the retina, the effective flow rate in that region is about twice this value. RBF increased during hypoxemia. (PaO2
