Four clones of Borrelia burgdorferi sensu stricto cause invasive infection in humans
Four clones of Borrelia burgdorferi sensu stricto cause invasive infection in humans
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DOI：
10.1128/iai.67.7.3518-3524.1999
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发表时间：
1999-07-01
影响因子：
3.1
通讯作者：
Luft, BJ
中科院分区：
文献类型：
--
作者：
Seinost, G;Dykhuizen, DE;Luft, BJ
莱姆病开始于蜱叮咬的部位，产生原发性感染，并在感染过程的早期将生物体传播到继发部位。在感染早期由螺旋体表达的主要外表面蛋白是外表面蛋白C（OspC）。在严格意义上的伯氏疏螺旋体中，OspC高度可变。根据序列差异性，ospC基因可分为21个主要的等位基因群。为了评估由ospC组定义的菌株差异是否与侵袭性和致病性相关，我们比较了来自蜱、来自原发性游走性红斑皮肤病变和来自次要部位（主要来自血液和脊髓液）的主要ospC组的频率分布。蜱的ospC组的频率分布显着不同，从主要网站，这反过来又显着不同，从次要网站。主要类群A、B、I和K在主要场所的频率高于蜱类，并且是在次要场所发现的唯一类群。我们定义了三种主要的ospC组：一种是常见的蜱，但很少会导致人类疾病，第二种是只导致蜱叮咬部位的局部感染，第三种是导致全身性疾病。所有系统性B.严格意义上的伯氏螺旋体感染与四种ospC群相关，这在莱姆病的诊断、治疗和预防中具有重要意义。
Lyme disease begins at the site of a tick bite, producing a primary infection with spread of the organism to secondary sites occurring early in the course of infection. A major outer surface protein expressed by the spirochete early in infection is outer surface protein C (OspC). In Borrelia burgdorferi sensu stricto, OspC is highly variable. Based on sequence divergence, alleles of ospC can be divided into 21 major groups. To assess whether strain differences defined by ospC group are linked to invasiveness and pathogenicity, we compared the frequency distributions of major ospC groups from ticks, from the primary erythema migrans skin lesion, and from secondary sites, principally from blood and spinal fluid. The frequency distribution of ospC groups from ticks is significantly different from that from primary sites, which in turn is significantly different from that from secondary sites. The major groups A, B, I, and K had higher frequencies in the primary sites than in ticks and were the only groups found in secondary sites. We define three categories of major ospC groups: one that is common in ticks but very rarely if ever causes human disease, a second that causes only local infection at the tick bite site, and a third that causes systemic disease. The finding that all systemic B. burgdorferi sensu stricto infections are associated with four ospC groups has importance in the diagnosis, treatment, and prevention of Lyme disease.