Draft genomic and transcriptome resources for marine chelicerate Tachypleus tridentatus
Draft genomic and transcriptome resources for marine chelicerate Tachypleus tridentatus
复制标题
海洋螯肢动物 Tachypleus tridentatus 的基因组和转录组资源草案
DOI：
10.1038/sdata.2019.29
复制
发表时间：
2019-02-26
期刊：
影响因子：
9.8
通讯作者：
Han, Jun Feng
中科院分区：
文献类型：
--
作者：
Liao, Yong Yan;Xu, Peng Wei;Han, Jun Feng
中国鲎（Tachypleus tridentatus）是一种古老的海洋节肢动物，其历史可追溯到中古生代，为细菌或真菌污染的检测提供了宝贵的资源。然而，对鲎变形细胞裂解物的过度开发已经大大减少了中国鲎的数量。因此，我们目前的测序，组装和注释的T。tridentatus，希望了解活化石的基因组特征，并协助科学家保护这一濒危物种。最终的基因组包含总大小为1.943 Gb，覆盖估计的基因组大小的90.23%。构建了三个幼虫期的转录组，以研究与幼虫发育相关的候选基因并验证注释。用BUSCO软件对基因组和基因模型的完整性进行了评估，分别达到96.2%和95.4%。旁系同源物的同义替换分布表明，T. tridentatus经历了两轮全基因组复制。所有的基因组和转录组数据都已存入公共数据库，供研究马蹄蟹的研究人员使用。
Chinese horseshoe crabs (Tachypleus tridentatus), ancient marine arthropods dating back to the mid-Palaeozoic Era, have provided valuable resources for the detection of bacterial or fungal contamination. However, excessive exploitation for the amoebocyte lysate of Tachypleus has dramatically decreased the population of the Chinese horseshoe crabs. Thus, we present sequencing, assembly and annotation of T. tridentatus, with the hope of understanding the genomic feature of the living fossil and assisting scientists with the protection of this endangered species. The final genome contained a total size of 1.943 Gb, covering 90.23% of the estimated genome size. The transcriptome of three larval stages was constructed to investigate the candidate gene involved in the larval development and validate annotation. The completeness of the genome and gene models was estimated by BUSCO, reaching 96.2% and 95.4%, respectively. The synonymous substitution distribution of paralogues revealed that T. tridentatus had undergone two rounds of whole-genome duplication. All genomic and transcriptome data have been deposited in public databases, ready to be used by researchers working on horseshoe crabs.