Preparation of GTPases for structural and biophysical analysis.
Preparation of GTPases for structural and biophysical analysis.
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用于结构和生物物理分析的 GTP 酶的制备。
DOI：
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发表时间：
2002
影响因子：
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通讯作者：
K. Rittinger
中科院分区：
文献类型：
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作者：
Susan J. Smith;K. Rittinger
在过去的10年里，研究人员已经确定了Rasrelated GTP酶在细胞生物学的几乎每个方面的关键作用。在20世纪80年代，原癌基因Ras本身是关注的焦点，尽管在20世纪90年代，这转移到越来越多的Ras相关蛋白质上。在这个新的十年里，还需要做很多工作来确定人类基因组计划现在发现的所有小GTP酶的作用。特别是，这些GTP酶需要在适当的生物化学和细胞背景下理解。在试图揭示这些多功能蛋白质的作用的过程中，已经开发了各种新的技术和方法。这些现在使研究人员能够轻松地在多种领域内移动，从结构研究到实时体内分析GTdR。由于认识到需要获得关键的背景方法，GTTRIPs Protocols：The Ras Superfamily致力于目前广泛使用的技术，并将继续成为全世界研究人员的标准。每一章的目的是提供详细的方法，使复制在任何实验室，同时也提供了一般原则上的方法是基于。第一部分中的一些技术广泛适用于小型GTP酶，而第二部分中的其他技术则更适用于每个GTP酶亚家族。我们要感谢许多作者花时间分享他们的专业知识。希望GTTRIP协议：Ras超家族将为所有从事新研究的人带来对这些非凡蛋白质的访问和理解。
In the last 10 years researchers have firmly established key roles for Rasrelated GTPases in almost every aspect of cell biology. In the 1980s the protooncogene Ras itself was the focus of interest, though in the 1990s this shifted to the increasing variety of Ras-related proteins. In this new decade much yet needs to be done to establish the role for all the small GTPases now uncovered by the human genome project. In particular, these GTPases need to be understood in the appropriate biochemical and cellular contexts. In the process of trying to uncover the role of these versatile proteins, a variety of novel techniques and methodologies has been developed. These now enable investigators to move easily within a diversity of fields ranging from structural studies to real-time in vivo analysis of a GTPase. In recognition of the need for access to key background methodologies, GTPase Protocols: The Ras Superfamily is devoted to techniques that are presently widely used and that will continue to be the standard for researchers worldwide. Each chapter is aimed at supplying detailed methodologies to allow reproduction in any laboratory, while also providing the general principles on which the methods are based. Some of the techniques grouped in the first section apply broadly to small GTPases, whereas others in Part II are more applicable within each GTPase subfamily. We would like to thank the many authors who have spent time in sharing their expertise. It is hoped that GTPase Protocols: The Ras Superfamily will bring access to and understanding of these remarkable proteins for all those embarking on new studies.