Differentiation of human NK cells into NK1 and NK2 subsets.
Differentiation of human NK cells into NK1 and NK2 subsets.
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DOI：
10.4049/jimmunol.161.11.5821
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发表时间：
1998-12
影响因子：
4.4
通讯作者：
David Peritt;Susan J. Robertson;G. Gri;Louise C. Showe;M. Aste‐Amezaga;Giorgio Trinchieri
中科院分区：
文献类型：
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作者：
David Peritt;Susan J. Robertson;G. Gri;Louise C. Showe;M. Aste‐Amezaga;Giorgio Trinchieri
在IL-12或IL-4存在下培养的人NK细胞分化成具有与Th 1和Th 2细胞相似的不同细胞因子分泌模式的细胞群。在IL-12（NK 1）中生长的NK细胞产生IL-10和IFN-γ，而在IL-4（NK 2）中生长的NK细胞产生IL-5和IL-13。虽然这些NK细胞亚群在细胞毒活性上没有差异，但NK 1细胞比NK 2细胞表达更高水平的细胞表面CD 95（Fas）Ag，并且对Ab或化学诱导的细胞凋亡更敏感。与Th 1细胞一样，NK 1细胞积累了更高水平的IL-12 R β 2链mRNA，并且在STAT 4转录因子的活化水平上比NK 2细胞对IL-12的反应性显著更高。与T细胞亚群类似的NK细胞亚群的鉴定表明NK细胞在先天性炎症反应及其对适应性免疫的影响中的新作用。
Human NK cells cultured in the presence of IL-12 or IL-4 differentiate into cell populations with distinct patterns of cytokine secretion similar to Th1 and Th2 cells. NK cells grown in IL-12 (NK1) produce IL-10 and IFN-gamma, whereas NK cells grown in IL-4 (NK2) produce IL-5 and IL-13. Although these NK cell subsets do not differ in cytotoxic activity, NK1 cells express higher levels of cell surface CD95 (Fas) Ag than NK2 cells and are more sensitive to Ab or chemically induced apoptosis. Like Th1 cells, NK1 cells accumulate much higher levels of the IL-12Rbeta2-chain mRNA and are significantly more responsive to IL-12 than NK2 cells at the level of activation of STAT4 transcription factor. The identification of NK cell subsets that are analogous to T cell subsets suggests a new role for NK cells in innate inflammatory responses and in their effect on adaptive immunity.