Genetic diversity and structure of the West Balkan Pramenka sheep types as revealed by microsatellite and mitochondrial DNA analysis
Genetic diversity and structure of the West Balkan Pramenka sheep types as revealed by microsatellite and mitochondrial DNA analysis
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DOI：
10.1111/j.1439-0388.2008.00742.x
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发表时间：
2008-12-01
影响因子：
2.6
通讯作者：
Kantanen, J.
中科院分区：
文献类型：
--
作者：
Cinkulov, M.;Popovski, Z.;Kantanen, J.
几种不同的表型的本地Pramenka羊已开发的巴尔干半岛的地区不同的环境和社会文化条件。从7个西巴尔干半岛的Pramenka羊类型的动物进行了分析15个微卫星标记和线粒体DNA（mtDNA）和结果被用来评估内和类型之间的遗传变异，并推断Pramenka羊的遗传群体结构。微卫星位点和绵羊类型的平均期望杂合度和等位基因丰富度分别为0.78和7.9。估计隐藏的遗传结构的贝叶斯统计方法表明，最大的panmictic群体的核心是由塞尔维亚，塞尔维亚，波斯尼亚，黑山和阿尔巴尼亚类型，而克罗地亚和马其顿类型分别包括两个其他主要群体。线粒体DNA分析表明，在普拉缅卡羊中存在两个mtDNA单倍型群，B和A，频率分别为93.7%和6.3%。在64只动物中共发现60种mtDNA单倍型，平均核苷酸差异为0.013，平均单倍型差异为0.945。分子分析表明，西巴尔干半岛的Pramenka绵羊类型有其起源于两个不同的母系家庭绵羊和不同的Pramenka表型往往形成几个panmictic人口。Pramenka羊代表了绵羊遗传多样性的宝贵资源。
Several different phenotypes of the native Pramenka sheep have been developed in the Balkan region for different environmental and socio-cultural conditions. Animals from seven West Balkan Pramenka sheep types were analysed for 15 microsatellite markers and for mitochondrial DNA (mtDNA) and the results were used to assess genetic variation within and among the types and to infer the genetic population structure of the Pramenka sheep. Mean expected heterozygosity and allelic richness over the microsatellite loci and sheep types were 0.78 and 7.9, respectively. A Bayesian statistical method for estimating hidden genetic structure suggested that a core of the largest panmictic population was formed by Serbian, Kosovan, Bosnian, Montenegrin and Albanian types, while Croatian and Macedonian types comprised two other main populations, respectively. Mitochondrial DNA analysis revealed two mtDNA haplogroups in the Pramenka sheep, B and A, with a frequency of 93.7% and 6.3%, respectively. A total of 60 mtDNA haplotypes were found in 64 animals sequenced, and the mean nucleotide and haplotypic diversities over the types were 0.013 and 0.945, respectively. Molecular analysis suggests that the West Balkan Pramenka sheep types have their origins in two distinct maternal lineages of domestic sheep and different Pramenka phenotypes tend to form few panmictic populations. The Pramenka sheep represents a valuable resource of genetic diversity in sheep.