Simultaneous Determination of Isollavones, Saponins and Flavones in Flos Puerariae by Ultra Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry
Simultaneous Determination of Isollavones, Saponins and Flavones in Flos Puerariae by Ultra Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry
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DOI：
10.1248/cpb.c13-00271
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发表时间：
2013-09-01
影响因子：
1.7
通讯作者：
Yuan, Dan
中科院分区：
文献类型：
--
作者：
Lu, Jing;Xie, Yuanyuan;Yuan, Dan
建立了超高效液相色谱(UPLC)和四极杆飞行时间质谱仪(QTOF/MS)联用技术，对葛根和葛花中的异黄酮类、皂苷和黄酮类成分进行了快速分析。样品萃取物与道地标共层析，比较保留时间、紫外光谱、特征分子离子和碎片离子与道地标样的保留时间、紫外光谱、特征分子离子和碎片离子，或用MS/MS结合MS Fragment软件进行初步鉴定，共鉴定出25个异黄酮类成分、13个皂苷和3个黄酮类成分。并对29种组分的同时定量进行了验证。两种葛花样品中异黄酮类、皂苷类和类黄酮类成分在质量和数量上存在较大差异，这为其化学成分的区分提供了可能，可作为其标准化和质量控制的依据。用主成分分析(PCA)和正交偏最小二乘判别分析(OPLS-DA)对UPLC-MS获得的数据进行处理，综合比较两种葛花之间的差异。
An ultra performance liquid chromatography (UPLC) coupled with quadrupole time-of-flight mass spectrometry (QTOF/MS) method is established for the rapid analysis of isoflavones, saponins and flavones in 16 samples originated from the flowers of Pueraria lobata and P. thomsonii. A total of 25 isoflavones, 13 saponins and 3 flavones were identified by co-chromatography of sample extract with authentic standards and comparing the retention time, UV spectra, characteristic molecular ions and fragment ions with those of authentic standards, or tentatively identified by MS/MS determination along with Mass Fragment software. Moreover, the method was validated for the simultaneous quantification of 29 components. The samples from two Pueraria flowers significantly differed in the quality and quantity of isoflavones, saponins and flavones, which allows the possibility of showing their chemical distinctness, and may be useful in their standardization and quality control. Dataset obtained from UPLC-MS was processed with principal component analysis (PCA) and orthogonal partial least squared discriminant analysis (OPLS-DA) to holistically compare the difference between both Pueraria flowers.