The Starfish Asterina pectinifera: Collection and Maintenance of Adults and Rearing and Metamorphosis of Larvae
The Starfish Asterina pectinifera: Collection and Maintenance of Adults and Rearing and Metamorphosis of Larvae
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海星海星：成虫的采集和维护以及幼虫的饲养和变态
DOI：
10.1007/978-1-0716-0974-3_3
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发表时间：
2021
影响因子：
--
通讯作者：
Takeo Kishimoto
中科院分区：
文献类型：
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作者：
Naoyuki Murabe;Ei-ichi Okumura;Kazuyoshi Chiba;Enako Hosoda;Susumu Ikegami;Takeo Kishimoto
在这里，我们描述了(a)在海星繁殖期收集海星的方法；(b)在实验室水族箱中饲养性腺发育完全的成虫；(c)将受精卵饲养成臂线虫幼虫，以及(d)在实验室条件下诱导幼虫蜕变成幼虫。这样的协议应该有助于在这些模式生物的整个生命周期中对海星发育进行各种分析。
Here we describe methods for (a) collecting starfish during their breeding period; (b) maintaining adults with fully grown gonads in laboratory aquaria; (c) rearing fertilized eggs to brachiolaria larvae, and (d) inducing larvae to metamorphose into juveniles under laboratory conditions. Such protocols should facilitate various analyses of starfish development throughout the entire life cycle of these model organisms.