Localization of the human tissue factor recognition determinant of human factor VIIa.
Localization of the human tissue factor recognition determinant of human factor VIIa.
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DOI：
10.1016/s0021-9258(18)54982-6
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发表时间：
1991-10
期刊：
影响因子：
--
通讯作者：
J. Toomey;K. Smith;D. Stafford
中科院分区：
文献类型：
--
作者：
J. Toomey;K. Smith;D. Stafford
组织因子是一种完整的膜糖蛋白，作为凝血因子via的必要辅助因子。最近的研究试图定位人因子VIIa的组织因子识别决定因素。虽然VIIa因子的几个区域被认为对组织因子结合很重要，但人类VIIa因子的高亲和力组织因子识别决定因素尚不清楚。为了确定决定因子，我们构建了一组由因子VII和因子IX部分组成的六种嵌合蛋白。然后，我们将嵌合体与125i标记的重组组织因子VIIa结合到Immobilon-P膜上进行竞争实验。结果表明，人因子VIIa的高亲和力组织因子识别决定因子位于表皮生长因子域内。
Tissue factor is an integral membrane glycoprotein that serves as an essential cofactor for the blood coagulation factor VIIa. Recent studies have attempted to localize the tissue factor recognition determinant of human factor VIIa. While several regions of factor VIIa have been implicated as important for tissue factor binding, the high affinity tissue factor recognition determinant of human factor VIIa is unknown. In order to define the determinant, we constructed a set of six chimeric proteins composed of portions of factor VII and factor IX. We then utilized the chimeras in competition experiments with 125I-labeled factor VIIa for recombinant tissue factor bound to an Immobilon-P membrane. The data indicate that the high affinity tissue factor recognition determinant of human factor VIIa is within the epidermal growth factor domains.