QTL mapping of resistance to Fusarium ear rot using a RIL population in maize
QTL mapping of resistance to Fusarium ear rot using a RIL population in maize
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DOI：
10.1007/s11032-008-9184-4
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发表时间：
2008-10-01
影响因子：
3.1
通讯作者：
Li, Jian-Sheng
中科院分区：
文献类型：
--
作者：
Ding, Jun-Qiang;Wang, Xiao-Ming;Li, Jian-Sheng
镰刀菌穗腐病是玉米上的一种流行病害，降低了玉米产量和品质。抗病育种是减少病害损失的有效途径。本研究以抗病（87-1）和感病（综3）亲本为材料，利用重组自交系群体沿着的187个株系，于2004年和2006年在郑州和北京进行了三次重复种植。使用钉穿孔法人工接种各品系。显着的基因型变异，在这两年检测到镰刀菌穗腐病。利用246个多态性SSR标记构建的平均遗传距离为9.1cM的遗传图谱，首次利用复合区间作图法（CIM）对小麦抗穗腐病QTL进行了分析。2004年在郑州和北京都检测到3个QTL，2006年分别检测到3个和4个QTL。抗病亲本贡献了所有的抗性QTL。利用复合区间作图和混合模型（MCIM），在不同环境中观察到镰刀菌穗腐病的上位性效应以及作图位点与环境之间的互作。两种方法在所有环境中一致地鉴定了3号染色体上的两个QTL（3.04 bin）。效应最大的主效抗性QTL位于第3染色体上的标记umc 1025和umc 1742之间（3.04 bin），解释了13-22%的表型变异。在玉米穗粒腐病主效抗性QTL两侧的SSR标记将有助于玉米穗粒腐病抗性的分子标记辅助选择。
Fusarium ear rot is a prevalent disease in maize, reducing grain yields and quality. Resistance breeding is an efficient way to minimize losses caused by the disease. In this study, 187 lines from a RIL population along with the resistant (87-1) and susceptible (Zong 3) parents were planted in Zhengzhou and Beijing with three replications in years 2004 and 2006. Each line was artificially inoculated using the nail-punch method. Significant genotypic variation in response to Fusarium ear rot was detected in both years. Based on a genetic map containing 246 polymorphic SSR markers with average genetic distances of 9.1 cM, the ear-rot resistance QTL were firstly analyzed by composite interval mapping (CIM). Three QTL were detected in both Zhengzhou and Beijing in 2004; and three and four QTL, respectively, were identified in 2006. The resistant parent contributed all resistance QTL. By using composite interval mapping and a mixed model (MCIM), significant epistatic effects on Fusarium ear rot as well as interactions between mapped loci and environments were observed across environments. Two QTL on chromosome 3 (3.04 bin) were consistently identified across all environments by the two methods. The major resistant QTL with the largest effect was flanked by markers umc1025 and umc1742 on chromosome 3 (3.04 bin), explaining 13-22% of the phenotypic variation. The SSR markers closely flanking the major resistance QTL will facilitate marker-assisted selection (MAS) of resistance to Fusarium ear rot in maize breeding programs.