A glucose electrode based on carbon paste chemically modified with a ferrocene-containing siloxane polymer and glucose oxidase, coated with a poly(ester-sulfonic acid) cation-exchanger
A glucose electrode based on carbon paste chemically modified with a ferrocene-containing siloxane polymer and glucose oxidase, coated with a poly(ester-sulfonic acid) cation-exchanger
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一种葡萄糖电极，基于用含二茂铁的硅氧烷聚合物和葡萄糖氧化酶化学改性的碳糊，涂有聚（酯磺酸）阳离子交换剂
DOI：
10.1016/s0003-2670(00)80474-2
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发表时间：
1990
影响因子：
6.2
通讯作者：
T. Skotheim
中科院分区：
文献类型：
--
作者：
L. Gorton;H. I. Karan;P. Hale;T. Inagaki;Y. Okamoto;T. Skotheim
通过用聚（酯磺酸）阳离子交换剂 Eastman AQ-29D 涂覆电极表面，对用葡萄糖氧化酶和含二茂铁的硅氧烷聚合物进行化学改性的碳糊进行进一步改性。聚合物以均匀的水分散体形式获得，pH 5-6；干燥后，聚合物涂层不溶于水。研究表明，该涂层不会损害酶活性，但可以防止电化学活性阴离子干扰物（例如抗坏血酸盐和尿酸盐）到达电极表面。聚合物涂层还可以防止葡萄糖氧化酶从碳糊中泄漏到接触溶液中，并保护电极表面免受尿液和牛血清白蛋白中存在的污垢剂的影响。未涂覆的电极在缓冲液中储存三周后，其对葡萄糖的原始响应损失了约 10-2-15%，而涂覆的电极的响应保持恒定。未涂层电极的葡萄糖校准曲线在高达约 5 mM 和涂层电极高达 20 mM 时均呈严格线性。涂覆后对葡萄糖的响应电流没有降低。
A carbon-paste chemically modified with glucose oxidase and a ferrocene-containing siloxane polymer was further modified by coating the electrode surface with a poly(ester-sulfonic acid) cation-exchanger, Eastman AQ-29D. The polymer is obtained as a homogeneous aqueous dispersion at pH 5–6; when dried, the polymer coating is not water-soluble. The coating was shown not to be detrimental to the enzyme activity but to prevent electrochemically active anionic interferents such as ascorbate and urate from reaching the electrode surface. The polymer coating also prevented glucose oxidase from leaking out of the carbon paste into the contacting solution and protected the electrode surface from fouling agents present in urine and bovine serum albumin. Uncoated electrodes lost some 10-2-15% of their original response to glucose after storage in buffer for three weeks whereas the response of the coated electrodes remained constant. Calibration curves for glucose were strictly linear up to about 5 mM for uncoated and up to 20 mM for coated electrodes. The response current to glucose was not decreased after coating.
影响因子：
7.4
作者：
KRISTENSEN, EW;KUHR, WG;WIGHTMAN, RM
通讯作者：
WIGHTMAN, RM