In vitro granuloma formation, NO production and cytokines profile from human mononuclear cells induced by fractionated antigens of Paracoccidioides brasiliensis
In vitro granuloma formation, NO production and cytokines profile from human mononuclear cells induced by fractionated antigens of Paracoccidioides brasiliensis
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DOI：
10.1016/s0198-8859(01)00268-3
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发表时间：
2001-08-01
期刊：
影响因子：
2.7
通讯作者：
Goes, AM
中科院分区：
文献类型：
--
作者：
Diniz, SN;Cisalpino, PS;Goes, AM
巴西副球孢子菌引起南美洲流行的慢性肉芽肿性真菌病，细胞介导的免疫是预防这种真菌感染的主要方式。我们在体外研究了表现不同临床形式的副球孢子菌病 (PCM) 患者的外周血单核细胞 (PBMC) 对巴西假单胞菌酵母细胞裂解物 (PbAg) 抗原组分的反应。这些指定为 F0 至 FV 的级分是在 FPLC 系统上使用阴离子交换色谱法获得的。我们的研究表明不同抗原组分诱导的细胞反应存在差异。 F0级分导致细胞增殖、肉芽肿形成显着减少，同时IL-10的产生显着升高。 FII和FIII级分增加了与高TNF-α产生相关的体外肉芽肿形成。除此之外，在急性 PCM 患者中，FII 和 FIII 会引起 NO 产生减少，但 F0 不会导致 NO 产生非常高。研究结果表明，P. brasiliensis 抗原成分参与 PCM 中 PBMC 反应的调节或激活，IL-10 和 NO 在体外肉芽肿形成的调节中可能很重要。人类免疫学 62, 799-808 (2001)。 (C) 美国组织相容性和免疫遗传学学会，2001 年。由 Elsevier Science Inc. 出版。
Paracoccidioides brasiliensis causes a chronic granulomatous mycosis prevalent in South America, and cell-mediated immunity represents the main mode of protection against this fungal infection. We investigated in vitro the response of peripheral blood mononuclear cells (PBMC) from paracoccidioidomycosis (PCM) patients presenting different clinical forms to antigenic fractions from P. brasiliensis yeast cell lysate (PbAg). These fractions designated F0 to FV were obtained using anion-exchange chromatography on a FPLC system. Our studies showed variation in the cellular responses induced by different antigenic fractions. The fraction F0 caused significant decrease in cellular proliferation, granuloma formation, accompanied by significant elevation in the production of IL-10. The fractions FII and FIII increased in vitro granuloma formation associated with high production of TNF-a. Besides that, FII and FIII evoked decrease in NO production but not F0 that induced very high levels, among patients with PCM from acute form. The findings suggest that P. brasiliensis antigenic components participate in the modulation or activation of PBMC response in PCM, and IL-10 and NO could be important in the regulation of in vitro granuloma formation. Human Immunology 62, 799-808 (2001). (C) American Society for Histocompatibility and Immunogenetics, 2001. Published by Elsevier Science Inc.