Peripheral blood CD4+ and CD8+ T cell type 1 and type 2 cytokine production in atopic asthmatic and normal subjects
Peripheral blood CD4+ and CD8+ T cell type 1 and type 2 cytokine production in atopic asthmatic and normal subjects
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DOI：
10.1046/j.1365-2222.2002.01281.x
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发表时间：
2002-03-01
影响因子：
6.1
通讯作者：
Johnston, SL
中科院分区：
文献类型：
--
作者：
Cho, SH;Stanciu, LA;Johnston, SL
背景：CD4(+)T细胞产生的IL-4和IL-5增加，产生的干扰素-γ减少，与哮喘的发病机制有关。但CD8(+)T细胞也产生I型和II型细胞因子，而CD4(+)和CD8(+)T细胞细胞因子在哮喘发病中的作用尚未见报道。目的检测哮喘患者和正常人外周血中CD8(+)和CD8(+)T细胞亚群分泌的I型和II型细胞因子。对24例特应性哮喘患者和20例正常人外周血中的CD8(+)T细胞和CD8(+)T细胞进行-13和干扰素-γ的检测。CD8(+)T细胞产生I型或II型细胞因子的能力。与正常对照组比较，哮喘组CD8(+)(20.0+/-2.9%)和CD8(+)(20.0+/-2.9%)显著升高(P<0.01)。
Background Increased production of IL-4 and IL-5 and decreased production of IFN-gamma by CD4(+) T cells has been implicated in asthma pathogenesis. However, CD8(+) T cells also produce type I and type 2 cytokines and the relative roles of CD4(+) and CD8(+) T cell cytokine production in asthma have not been previously studied.Objective To determine the production of the type I and type 2 cytokines by CD4(+) and CD8(+) T cell subsets in asthmatic and normal subjects.Methods Intracellular cytokine staining for IL-4, -5, -10, -13 and IFN-gamma was analysed in peripheral blood CD4(+) and CD8(+) T cells from 24 atopic asthmatic and 20 normal subjects.Results Both subsets of T cells produced all cytokines studied and there were no significant differences between CD4(+). and CD8(+) T cells in their capacity to produce either type I or type 2 cytokines. There were significantly increased frequencies of IFN-gamma-positive CD4(+) (13.1 +/- 2.4%, vs. 7.3 +/- 1.4%) and CD8(+) (20.0 +/- 2.9%, vs. 9.6 +/- 2.1%) T cells in asthmatic subjects compared with normal subjects (P