Evaluation of different culture systems with low oxygen tension on the development, quality and oxidative stress-related genes of bovine embryos produced in vitro
Evaluation of different culture systems with low oxygen tension on the development, quality and oxidative stress-related genes of bovine embryos produced in vitro
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DOI：
10.1017/s0967199411000025
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发表时间：
2012-08-01
期刊：
影响因子：
1.7
通讯作者：
Felmer, Ricardo
中科院分区：
文献类型：
--
作者：
Elena Arias, Maria;Sanchez, Raul;Felmer, Ricardo
本研究旨在探讨不同低氧培养体系（5%CO2、5%O-2和90%N-2）对牛胚胎发育、质量和氧化应激相关基因表达谱的影响。评估的系统包括：（1）孵育室;（2）塑料袋;和（3）铝箔袋。培养体系的选择对72 h卵裂率无影响。但在第7天的囊胚登记率方面，观察到了显著差异（P < 0.01）（培养箱、塑料袋和铝箔袋分别为29.8%、20.2%和12.7%）。细胞总数在系统之间没有差异，尽管ICM：总细胞的比例受到影响，特别是在塑料袋中（19.5%），与孵育室（31.4%）相比。此外，发现凋亡：总细胞比率存在显著差异（孵育室、塑料袋和铝箔袋分别为3.3%、6.5%和8.8%），凋亡细胞核主要位于胚胎的ICM隔室中。培养系统之间的活性氧簇的量也不同，这种效果与在充气袋中培养的胚胎中SOD 2，GSS和GPX 1基因的较高表达相关，与在孵化器室中培养的胚胎相比。总之，这些结果证明，在低氧张力下，孵化室更有效，产生更多的，更好的质量，胚胎比充气袋系统在这里评估，这种效果可能是由于活性氧在充气袋中的水平增加，上调一些抗氧化酶的表达，以补偿高氧条件。
The present study was conducted to assess the development, quality and gene expression profile of oxidative stress-related genes of bovine embryos cultured in different culture systems with low oxygen tension (5% CO2, 5% O-2 and 90% N-2). The systems assessed included: (1) an incubator chamber; (2) a plastic bag; and (3) a foil bag. The choice of culture system had no effect on cleavage rate at 72 h. However, significant differences (P < 0.01) were observed in the rate of blastocysts registered at day 7 (29.8, 20.2 and 12.7% for incubator chamber, plastic bag and foil bag, respectively). Total number of cells did not differ between systems, although the proportion of ICM:total cells was affected particularly in the plastic bag (19.5%), compared with the incubator chamber (31.4%). In addition, significant differences were found in the apoptotic:total cell ratio (3.3, 6.5 and 8.8% for the incubator chamber, plastic bag and foil bag, respectively), with apoptotic nuclei localised mainly in the ICM compartment of the embryo. The amount of reactive oxygen species was also different between culture systems and this effect was correlated with a higher expression of SOD2,GSS and GPX1 genes in embryos cultured in the gassed bags as compared with embryos cultured in the incubator chamber. In conclusion, these results give evidence that, under low oxygen tension, the incubator chamber is more efficient and generates higher number of, and better quality, embryos than gassed bag systems evaluated here and this effect was probably due to an increased level of reactive oxygen species in the gassed bags, which upregulates the expression of some antioxidant enzymes to compensate for hyperoxia conditions.