Surface and biological evaluation of hydroxyapatite-based coatings on titanium deposited by different techniques
Surface and biological evaluation of hydroxyapatite-based coatings on titanium deposited by different techniques
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DOI：
10.1002/jbm.1065
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发表时间：
2001-12-05
期刊：
影响因子：
--
通讯作者：
Milella, E
中科院分区：
文献类型：
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作者：
Massaro, C;Baker, MA;Milella, E
采用等离子喷涂、溶胶-凝胶和溅射技术在纯钛表面制备了羟基磷灰石涂层，并应用于牙种植体。后两种技术是当前感兴趣的，因为它们允许沉积微米尺寸的涂层。采用扫描电子显微镜（SEM）、X射线光电子能谱（XPS）和X射线衍射（XRD）对涂层的形貌、成分和结构进行了研究。所有涂层均为均匀的，并表现出适合植入物应用的粗糙形态。溅射（退火后），等离子体喷涂，和溶胶-凝胶涂层都显示出对应于羟基磷灰石的衍射峰。观察到不同涂层类型的表面污染物不同。溅射涂层被发现具有最相似的羟基磷灰石的组合物，溶胶-凝胶沉积物也表现出高浓度的氢氧根离子。观察到的等离子喷涂涂层中的Ca/P比的差异，和碳酸根离子的小浓度被发现在电沉积涂层。使用MG 63成骨细胞样细胞的体外细胞培养研究证明了细胞在测试材料上增殖的能力。与溅射和等离子喷涂涂层相比，溶胶-凝胶涂层促进更高的细胞生长、更大的碱性磷酸酶活性和更大的骨钙素产生。(C)John Wiley & Sons，Inc.
Hydroxyapatite coatings have been deposited on titanium cp by plasma spray, sol-gel, and sputtering techniques for dental implant applications. The latter two techniques are of current interest, as they allow coatings of micrometer dimensions to be deposited. Coating morphology, composition, and structure have been investigated by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD). All coatings were homogeneous and exhibited a rough morphology suitable for implant applications. The sputtered (after annealing), plasma spray, and sol-gel coatings all showed diffraction peaks corresponding to hydroxyapatite. The surface contaminants were observed to be different for the different coating types. The sputtered coatings were found to have a composition most similar to hydroxyapatite; the sol-gel deposits also showed a high concentration of hydroxyl ions. A discrepancy in the Ca/P ratio was observed for the plasma spray coatings, and a small concentration of carbonate ions was found in the sputter-deposited coatings. The in vitro cell-culture studies using MG63 osteoblast-like cells demonstrated the ability of cells to proliferate on the materials tested. The sol-gel coating promotes higher cell growth, greater alkaline phosphatase activity, and greater osteocalcin production compared to the sputtered and plasma-sprayed coatings. (C) 2001 John Wiley & Sons, Inc.