Transferrin and iron release from rat hepatocytes in culture.
Transferrin and iron release from rat hepatocytes in culture.
复制标题
培养中的大鼠肝细胞释放转铁蛋白和铁。
DOI：
10.1152/ajpgi.1985.248.1.g93
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
E. Morgan
中科院分区：
文献类型：
--
作者：
E. Baker;M. Page;E. Morgan
The regulation of transferrin and iron release from the liver was studied using adult rat hepatocytes in primary monolayer culture. The cells were prelabeled by incubation with rat transferrin doubly labeled with iodine-125 and iron-59. Approximately 50% of the 125I-transferrin but only 10% of the iron-59 taken up by the cells was released during reincubation for 24 h. Less than 10% of the refluxed transferrin was catabolized as indicated by the protein-free iodine-125 values. These results suggest that at least part of iron uptake by hepatocytes is mediated by the reversible binding of transferrin in a manner comparable with erythroid cells and placenta. However, several iron chelators mobilized hepatic iron, in contrast to erythroid cells. Apotransferrin and desferrioxamine released a maximum of about 20% iron-59 with little effect on transferrin binding. A greater proportion of the iron-59 was available for chelation after shorter uptake times (1-2 h) than longer times. Hence, there are at least three iron compartments in hepatocytes in culture: rapidly refluxing iron that may be transferrin bound, a fixed pool, and a chelatable pool that may represent iron in transit between plasma transferrin and ferritin.
