Molecular genetic mapping of the mouse male sterility and histoincompatibility (mshi) mutation on proximal chromosome 10.
Molecular genetic mapping of the mouse male sterility and histoincompatibility (mshi) mutation on proximal chromosome 10.
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小鼠雄性不育和近端 10 号染色体上组织不相容性 (mshi) 突变的分子遗传图谱。
DOI：
10.1006/geno.1996.4475
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
King,TR
中科院分区：
文献类型：
--
作者：
Turner,JP;Carpentino,JE;Cantwell,AM;Hildebrandt,AL;Myrie,KA;King,TR
小鼠的隐性雄性不育和组织不相容性（mshi）突变对组织相容性和雄性生殖产生多效性效应，而雌性突变体似乎生殖正常。通过对126个种内回交后代的分析，我们将该突变定位于小鼠第10号染色体（Chr）。我们的分析都地方的雄性不育和组织不亲和性控制mshi之间的标记D10 Mit 51/D10 Mit 212和D10 Mit 170之间的20 cM的间隔，并允许其他几个微卫星标记Chr 10的顺序，以前没有解决。我们所描述的高分辨率回交面板应便于分离更紧密连接的探针序列，并最终对受此有趣突变影响的基因或基因进行分子鉴定。
The recessive male sterility and histoincompatibility (mshi) mutation in the mouse generates pleiotropic effects on histocompatibility and male reproduction, while female mutants appear to be reproductively normal. We have mapped themshimutation to mouse Chromosome (Chr) 10 by analysis of 126 progeny from an intraspecific backcross. Our analysis both places the male sterility and histoincompatibility controlled bymshiwithin a 20-cM interval between the markersD10Mit51/D10Mit212andD10Mit170and has allowed the ordering of several other microsatellite markers on Chr 10 that were previously unresolved. The high-resolution backcross panel we describe should facilitate the isolation of more tightly linked probe sequences and, ultimately, the molecular identification of the gene or genes affected by this interesting mutation.