Measurement of essential physical properties of vitrification solutions
Measurement of essential physical properties of vitrification solutions
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DOI：
10.1016/j.theriogenology.2006.09.029
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发表时间：
2007-01-01
期刊：
影响因子：
2.8
通讯作者：
Arav, A.
中科院分区：
文献类型：
--
作者：
Yavin, S.;Arav, A.
玻璃化冷冻是近年来发展迅速的一种“无冰”冷冻保存方法，有可能成为卵母细胞冷冻保存的首选方法。当试图通过玻璃化冷冻成功地保存卵母细胞时，应考虑五个损伤来源：溶液效果2。结晶3。4.玻璃骨折。脱硝和再结晶冷冻损伤玻璃化成功的概率取决于三个主要因素：样品的粘度；降温和升温速率；还有样品体积。与玻璃化液相关的问题之一是它可能含有高浓度的冷冻保护剂（CP），这可能通过化学毒性和渗透休克损害细胞。在本研究中，我们检查了与成功玻璃化相关的主要参数，并试图为玻璃化过程的最重要方面撰写指南。第一步是选择合适且毒性最小的玻璃化溶液。然后我们评估了冷却速率和体积对玻璃化概率的影响。减少样品体积，结合加速冷却，使CP浓度降低。然而，在实践中，必须在影响玻璃化概率的所有因素之间保持微妙的平衡，以防止结晶、反玻璃化、再结晶、玻璃断裂和冷伤。英国皇家版权所有(c) 2006出版的爱思唯尔公司。版权所有。
Vitrification is an "ice-free" cryopreservation method that has rapidly developed in recent years and might become the method of choice for oocyte cryopreservation. Five sources of damage should be considered when attempting to achieve successful oocyte cryopreservation by vitrification:1. Solution effects 2. Crystallization 3. Glass fractures 4. Devitrification and recrystallization 5. Chilling injuryThe probability of successful vitrification depends on three major factors: viscosity of the sample; cooling and warming rates; and sample volume. One of the problems associated with the vitrification solution is that it may contain high concentrations of cryoprotectants (CP), which can damage the cells through chemical toxicity and osmotic shock. In the present study, we examined the principal parameters associated with successful vitrification, and attempted to compose guidelines to the most important aspects of the vitrification process.The first step was the selection of a suitable and least toxic vitrification solution. We then evaluated the effects of cooling rate and volume on the probability of vitrification. Reduction of the sample volume, combined with accelerated cooling, enabled reduction of the CP concentration. However, in practice, a delicate balance must be maintained among all the factors that affect the probability of vitrification in order to prevent crystallization, devitrification, recrystallization, glass fractures and chilling injury. Crown Copyright (c) 2006 Published by Elsevier Inc. All rights reserved.