Comparative transcriptome analysis provides new insights into erect and prostrate growth in bermudagrass (Cynodon dactylon L.).
Comparative transcriptome analysis provides new insights into erect and prostrate growth in bermudagrass (Cynodon dactylon L.).
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DOI：
10.1016/j.plaphy.2017.10.016
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发表时间：
2017-12
期刊：
影响因子：
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通讯作者：
Bing Zhang;Xiaolin Xiao;Junqin Zong;Jingbo Chen;Jianjian Li;Hailin Guo;Jianxiu Liu
中科院分区：
文献类型：
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作者：
Bing Zhang;Xiaolin Xiao;Junqin Zong;Jingbo Chen;Jianjian Li;Hailin Guo;Jianxiu Liu
狗牙根（Cynodon dactylonL.）是一种重要的暖季型草坪和饲料草品种，具有多种用途。在大多数C。在栽培种和品种中，直立生长的茎（嫩枝）和匍匐生长的茎（匍匐茎）常常共存。这两种类型的茎都是通过分蘖形成的，但在两个方向上生长，具有不同的分蘖角度。阐明狗牙根分蘖角度的调控机制，可为选育不同株型的品种提供重要线索。在这项研究中，我们比较了茎节间转录组的两个狗牙根野生种质具有极其不同的分蘖角度和茎生长方向。共有2088个和12,141个unigenes分别在prostrate-growing wild accession C792和erect-growing wild accession C793中优先表达。京都基因和基因组百科全书（KEGG）基于同源性的注释系统（KOBAS）分析进一步表明，光和重力响应基因在C792中富集，而木质素合成相关基因在C793中富集，这很好地解释了两个种质中维管束和机械组织木质化的差异。这些结果不仅拓宽了对狗牙根分蘖角度和茎生长方向的遗传控制的认识，而且为狗牙根的分子育种提供了参考。鸭茅和其他具有不同植物结构的草坪草物种。
Bermudagrass (Cynodon dactylonL.) is a prominent warm-season turf and forage grass species with multiple applications. In mostC. dactyloncultivars and accessions, erect-growing stems (shoot) and prostrate-growing stems (stolon) often coexist. These two types of stems are both formed through tillering but grow in two directions with different tiller angles. Elucidating the mechanism of tiller angle regulation in bermudagrass could provide important clues to breed cultivars with different plant architectural features for diverse usage. In this study, we compared the stem internode transcriptome of two bermudagrass wild accessions with extremely different tiller angles and stem growth directions. A total of 2088 and 12,141 unigenes were preferentially expressed in prostrate-growing wild accession C792 and erect-growing wild accession C793, respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) Orthology-based Annotation System (KOBAS) analyses further indicated that light- and gravity-responsive genes were enriched in accession C792, whereas lignin synthesis-related genes were enriched in accession C793, which well explains the difference in lignification of vascular bundles and mechanical tissues in the two accessions. These results not only expand our understanding of the genetic control of tiller angle and stem growth direction in bermudagrass but also provide insight for future molecular breeding ofC. dactylonand other turfgrass species with different plant architectures.