BMP/SMAD signaling regulates the cell behaviors that drive the initial dorsal-specific regional morphogenesis of the otocyst.
BMP/SMAD signaling regulates the cell behaviors that drive the initial dorsal-specific regional morphogenesis of the otocyst.
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DOI：
10.1016/j.ydbio.2010.09.002
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发表时间：
2010-11-15
影响因子：
2.7
通讯作者：
Schoenwolf GC
中科院分区：
文献类型：
--
作者：
Ohta S;Mansour SL;Schoenwolf GC
在耳囊发育过程中，区域形态发生建立了前庭背腔和腹侧听腔，它们共同构成内耳的膜性迷路。我们确定了前庭形成的最早的形态发生事件，即耳囊背外侧壁的快速变薄和扩张，并表明这一过程是由耳囊细胞形状从柱状到鳞状的变化而产生的，而不是其他细胞行为的变化，如细胞增殖或细胞死亡的局部性变化。此外，我们还发现BMP/SMAD信号调节耳囊背外侧变薄和扩张，这是局部变薄和扩张的充分和必要的条件。最后，我们发现BMP/SMAD信号导致E-钙粘蛋白在耳囊背外侧的碎裂，并伴随着细胞形状的改变，提示BMP/SMAD信号在耳囊上皮细胞的初始形态发生过程中调节细胞与细胞的黏附。总之，我们的结果表明，通过Smads的BMP信号调节了驱动耳囊最初背部特异性形态发生的细胞行为，为复杂的器官雏形--发育中的膜迷路--的局部形态发生提供了新的信息。
During development of the otocyst, regional morphogenesis establishes a dorsal vestibular chamber and a ventral auditory chamber, which collectively constitute the membranous labyrinth of the inner ear. We identified the earliest morphogenetic event heralding the formation of the vestibular chamber, a rapid thinning and expansion of the dorsolateral wall of the otocyst, and showed that this process is generated by changes in otocyst cell shape from columnar to squamous, as opposed to changes in other cell behaviors, such as localized changes in cell proliferation or cell death. Moreover, we showed that thinning and expansion of the dorsolateral otocyst is regulated by BMP/SMAD signaling, which is both sufficient and necessary for localized thinning and expansion. Finally, we showed that BMP/SMAD signaling causes fragmentation of E-cadherin in the dorsolateral otocyst, occurring concomitantly with cell-shape change, suggesting that BMP/SMAD signaling regulates cell-cell adhesion during the initial morphogenesis of the otocyst epithelium. Collectively, our results show that BMP signaling via SMADs regulates the cell behaviors that drive the initial dorsal-specific morphogenesis of the otocyst, providing new information about how regional morphogenesis of a complex organ rudiment, the developing membranous labyrinth, is initiated.
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BMP4对于检测角头运动的前庭设备的形成至关重要。
影响因子：
4.5
作者：
Chang, Weise;Lin, Zhengshi;Kulessa, Holger;Hebert, Jean;Hogan, Brigid L. M.;Wu, Doris K.
通讯作者：
Wu, Doris K.
影响因子：
2.7
作者：
Chang, WS;Nunes, FD;Wu, DK
通讯作者：
Wu, DK
影响因子：
4.6
作者：
Chang, W;Brigande, JV;Wu, DK
通讯作者：
Wu, DK
影响因子：
3.7
作者：
Hammond, Katherine L.;Loynes, Helen E.;Whitfield, Tanya T.
通讯作者：
Whitfield, Tanya T.
DOI：
10.1002/neu.20171
发表时间：
2005-09-15
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
--
作者：
Dabdoub, A;Kelley, MW
通讯作者：
Kelley, MW