The estrous cycle of the ewe is resistant to disruption by repeated, acute psychosocial stress.
The estrous cycle of the ewe is resistant to disruption by repeated, acute psychosocial stress.
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母羊的发情周期能够抵抗反复的、剧烈的社会心理压力的干扰。
DOI：
10.1095/biolreprod.109.078774
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发表时间：
2010
影响因子：
3.6
通讯作者：
Karsch,FredJ
中科院分区：
文献类型：
--
作者：
Wagenmaker,ElizabethR;Breen,KellieM;Oakley,AmyE;Tilbrook,AlanJ;Karsch,FredJ
通过五个实验来验证心理社会应激干扰绵羊发情周期的假设。在实验1中，母羊在卵泡期被反复隔离。排卵前促黄体激素（LH）峰的时间、幅度和持续时间不受影响。在实验2中，卵泡期母羊进行了两次“分层应力”的范例组成的顺序，每小时应用程序的隔离，约束，蒙眼，和捕食者的线索。这降低了LH脉冲幅度，但不影响LH浪涌。在实验3中，不同的急性应激源依次在卵泡期：食物拒绝加上不熟悉的噪音和强迫运动，分层的压力，午夜前后的运动，和运输。这也没有影响LH激增。在实验4中，在两个完整的发情周期中，每1-2天给予可变的急性心理社会应激;这不会破坏监测的周期的任何参数。最后，实验5研究了如果绵羊受到慢性限食的代谢应激，实验4的心理社会应激范式是否会破坏周期和发情行为。百分之三十的食物限制母羊表现出恶化的发情周期参数，然后停止周期和未能表达发情行为。然而，对于同样遇到心理社会压力的母羊来说，干扰并不更加明显。总的来说，这些研究结果表明，绵羊的发情周期是显着的抗中断的急性发作的心理压力间歇性地施加在一个单一的卵泡期或重复超过两个发情周期。
Five experiments were conducted to test the hypothesis that psychosocial stress interferes with the estrous cycle of sheep. In experiment 1, ewes were repeatedly isolated during the follicular phase. Timing, amplitude, and duration of the preovulatory luteinizing hormone (LH) surge were not affected. In experiment 2, follicular-phase ewes were subjected twice to a “layered stress” paradigm consisting of sequential, hourly application of isolation, restraint, blindfold, and predator cues. This reduced the LH pulse amplitude but did not affect the LH surge. In experiment 3, different acute stressors were given sequentially within the follicular phase: food denial plus unfamiliar noises and forced exercise, layered stress, exercise around midnight, and transportation. This, too, did not affect the LH surge. In experiment 4, variable acute psychosocial stress was given every 1–2 days for two entire estrous cycles; this did not disrupt any parameter of the cycle monitored. Lastly, experiment 5 examined whether the psychosocial stress paradigms of experiment 4 would disrupt the cycle and estrous behavior if sheep were metabolically stressed by chronic food restriction. Thirty percent of the food-restricted ewes exhibited deterioration of estrous cycle parameters followed by cessation of cycles and failure to express estrous behavior. However, disruption was not more evident in ewes that also encountered psychosocial stress. Collectively, these findings indicate the estrous cycle of sheep is remarkably resistant to disruption by acute bouts of psychosocial stress applied intermittently during either a single follicular phase or repeatedly over two estrous cycles.