In Vivo Deep-Brain Structural and Hemodynamic Multiphoton Microscopy Enabled by Quantum Dots
In Vivo Deep-Brain Structural and Hemodynamic Multiphoton Microscopy Enabled by Quantum Dots
复制标题
量子点实现的体内深部脑结构和血流动力学多光子显微镜
DOI：
10.1021/acs.nanolett.9b01708
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发表时间：
2019-08-01
期刊：
影响因子：
10.8
通讯作者：
Wang, Ke
中科院分区：
文献类型：
--
作者：
Liu, Hongji;Deng, Xiangquan;Wang, Ke
可视化脑深部血管和血液动力学是理解脑生理学和病理学的关键。在各种采用的成像方式，多光子显微镜（MPM）是众所周知的，其脑深部结构和血流动力学成像能力。然而，MPM的最大成像深度受到脑深部信号耗尽的限制。在这里，我们证明了量子点是一种使材料显着更深的结构和血液动力学MPM在小鼠大脑中的体内。我们表征了量子点的三光子激发和发射参数：在1700 nm窗口处测量的量子点的三光子截面比常规荧光染料的三光子截面大4-5个数量级，而在体内循环血液中测量的三光子发射光谱与离体测量相比显示出轻微的红移和加宽。在这些测量结果的基础上，我们进一步证明了结构和血液动力学的三光子显微镜在小鼠大脑中的量子点标记的体内，在所有MPM模式在所有演示的激发波长之间的记录深度。
Visualizing deep-brain vasculature and hemodynamics is key to understanding brain physiology and pathology. Among the various adopted imaging modalities, multiphoton microscopy (MPM) is well-known for its deep-brain structural and hemodynamic imaging capability. However, the largest imaging depth in MPM is limited by signal depletion in the deep brain. Here we demonstrate that quantum dots are an enabling material for significantly deeper structural and hemodynamic MPM in mouse brain in vivo. We characterized both three-photon excitation and emission parameters for quantum dots: the measured three-photon cross sections of quantum dots are 4-5 orders of magnitude larger than those of conventional fluorescent dyes excited at the 1700 nm window, while the three-photon emission spectrum measured in the circulating blood in vivo shows a slight red shift and broadening compared with ex vivo measurement. On the basis of these measured results, we further demonstrate both structural and hemodynamic three-photon microscopy in the mouse brain in vivo labeled by quantum dots, at record depths among all MPM modalities at all demonstrated excitation wavelengths.