Placental-specific microRNA in maternal circulation - identification of appropriate pregnancy-associated microRNAs with diagnostic potential
Placental-specific microRNA in maternal circulation - identification of appropriate pregnancy-associated microRNAs with diagnostic potential
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DOI：
10.1016/j.jri.2011.02.006
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发表时间：
2011-05-01
影响因子：
3.4
通讯作者：
Hromadnikova, Ilona
中科院分区：
文献类型：
--
作者：
Kotlabova, Katerina;Doucha, Jindrich;Hromadnikova, Ilona
本研究的目的是确定母体血浆中存在的胎盘特异性 microRNA，以区分正常妊娠的女性和非妊娠个体。选择具有诊断潜力的适当妊娠相关 microRNA 的标准如下：(1) 足月胎盘中的检出率为 100%，(2) 整个妊娠期间母体血浆中的检出率≥ 67%（6 个测试孔中至少有 4 个阳性孔），以及 (3) 在非妊娠个体的全外周血和血浆样本中未检出。最初，我们测试了 microRNA（miR-34c、miR-372、miR-135b 和 miR-518b），这些 microRNA 之前已被确定为妊娠相关 microRNA。此外，我们选择了其他 16 种高度特异性胎盘 microRNA（miR-512-5p、miR-515-5p、miR-224、miR-516-5p、miR-517*、miR-136、miR-518f*、miR-519a、miR-519d、miR-519e、miR-520a*、 miR-520h、miR-524-5p、miR-525、miR-526a 和 miR-526b) 来自 miRNAMap 数据库。 7 个 microRNA（miR-516-5p、miR-517*、miR-518b、miR-520a*、miR-520h、miR-525 和 miR-526a）被鉴定为具有诊断潜力的新妊娠相关 microRNA。妊娠第 36 周期间，它们在母体血浆中的水平相当于每毫升母体血浆 45.0-427.0 pg 总 RNA（富含小 RNA）。 (C) 2011 Elsevier Ireland Ltd. 保留所有权利。
The goal of this study was to identify placental specific microRNAs present in maternal plasma that differentiate between women with normal pregnancies and nonpregnant individuals. The selection of appropriate pregnancy-associated microRNAs with diagnostic potential was based on the following criteria: (1) detection rate of 100% in full-term placentas, (2) detection rate of >= 67% in maternal plasma throughout gestation (at least four positive wells out of six tested wells) and (3) not detectable in whole peripheral blood and plasma samples of nonpregnant individuals. Initially, we tested microRNAs (miR-34c, miR-372, miR-135b and miR-518b), which had been previously identified as pregnancy-associated microRNAs. Additionally, we selected 16 other highly specific placental microRNAs (miR-512-5p, miR-515-5p, miR-224, miR-516-5p, miR-517*, miR-136, miR-518f*, miR-519a, miR-519d, miR-519e, miR-520a*, miR-520h, miR-524-5p, miR-525, miR-526a, and miR-526b) from the miRNAMap database. Seven microRNAs (miR-516-5p, miR-517*, miR-518b, miR-520a*, miR-520h, miR-525 and miR-526a) were identified as new pregnancy associated microRNAs with diagnostic potential. Their levels in maternal plasma during the 36th week of gestation corresponded to 45.0-427.0 pg of total RNA (enriched for small RNAs) per milliliter of maternal plasma. (C) 2011 Elsevier Ireland Ltd. All rights reserved.