DELETION MUTANTS OF BACTERIOPHAGE LAMBDA .1. ISOLATION AND INITIAL CHARACTERIZATION
DELETION MUTANTS OF BACTERIOPHAGE LAMBDA .1. ISOLATION AND INITIAL CHARACTERIZATION
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DOI：
10.1016/0022-2836(71)90471-2
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发表时间：
1971-01-01
影响因子：
5.6
通讯作者：
HUSKEY, RJ
中科院分区：
文献类型：
--
作者：
PARKINSON, JS;HUSKEY, RJ
λ噬菌体在高温或低离子强度下的失活率很大程度上取决于λ头的DNA含量。因此，通过分离λ野生型的耐热或螯合剂抗性突变体来选择λ的缺失突变体。由于 λ 野生型种群含有约 0.01% 的表型抗性噬菌体，因此有必要通过回收程序来选择删除突变体，该程序似乎针对某些类型的删除进行选择。缺失已从紫外线诱导和营养生长的 λ 储备中分离出来，两种制剂在几个方面有所不同。根据初步表征的结果，所有缺失突变体根据其在不同宿主菌株中的整合行为分为三大类。此外，所研究的近 200 个缺失突变体中没有一个在促进无重组宿主细菌中的重组能力方面存在缺陷。
The inactivation rate of lambda phage at high temperature or low ionic strength is largely dependent on the DNA content of the λ head. Deletion mutants of λ have been selected, therefore, by isolating heat-resistant or chelating-agent-resistant mutants of λ wild type. Because λ wild-type stocks contain about 0.01% phenotypically resistant phage, it was necessary to select deletion mutants by recycling procedures which appear to select against certain types of deletions. Deletions have been isolated from ultraviolet-induced and vegetatively grown λ stocks and the two preparations are different in several respects. Based on the results of preliminary characterizations, all of the deletion mutants fall into three major classes with respect to their integration behavior in different host strains. Furthermore, none of the nearly 200 deletion mutants studied is defective in the ability to promote recombination in recombinationless host bacteria.