PENETRATION OF TOXOPLASMA-GONDII INTO HOST-CELLS INDUCES CHANGES IN THE DISTRIBUTION OF THE MITOCHONDRIA AND THE ENDOPLASMIC-RETICULUM
PENETRATION OF TOXOPLASMA-GONDII INTO HOST-CELLS INDUCES CHANGES IN THE DISTRIBUTION OF THE MITOCHONDRIA AND THE ENDOPLASMIC-RETICULUM
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DOI：
10.1247/csf.17.311
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发表时间：
1992-10-01
影响因子：
1.5
通讯作者：
DESOUZA, W
中科院分区：
文献类型：
--
作者：
DEMELO, EJT;DECARVALHO, TU;DESOUZA, W
用荧光显微镜和透射电子显微镜分析弓形虫与宿主细胞相互作用过程中，线粒体、内质网和高尔基复合体的荧光显微镜和透射电子显微镜在这些结构的组织结构上发生的变化。在未感染的细胞中，线粒体是长丝状结构，从核区向细胞外围辐射。在寄生虫穿透后，它们变短，并倾向于集中在位于寄主细胞细胞质中的含有寄生虫的液泡(寄生虫液泡)周围。胞外寄生虫的线粒体也被罗丹明123染色，但位于寄生虫液泡内的线粒体不被染色。DiOC6与内质网的成分结合，结合透射电子显微镜进行标记，发现寄生性液泡周围有密集的这种结构。排列在液泡内的膜也被染色，这表明内质网的成分也被结合到这个膜上。通过NBD-神经酰胺染色和电子显微镜观察发现，高尔基复合体在细胞内寄生的整个进化过程中保持其核周位置。
Fluorescence microscopy, using dyes which specifically label mitochondria, endoplasmic reticulum and the Golgi complex, and transmission electron microscopy, were used to analyze the changes which occur in the organization of these structures during interaction of Toxoplasma gondii with host cells. In uninfected cells the mitochondria are long filamentous structures which radiate from the nuclear region toward the cell periphery. After parasite penetration they become shorter and tend to concentrate around the parasite-containing vacuole (parasitophorous vacuole) located in the cytoplasm of the host cell. The mitochondria of extracellular parasites, but not of those located within the parasitophorous vacuole, were also stained by rhodamine 123. Labeling with DiOC6, which binds to elements of the endoplasmic reticulum, in association with transmission electron microscopy, revealed a concentration of this structure around the parasitophorous vacuole. The membrane lining this vacuole was also stained, suggesting that components of the endoplasmic reticulum are also incorporated into this membrane. The Golgi complex, as revealed by staining with NBD-ceramide and electron microscopy, maintains its perinuclear position throughout the evolution of the intracellular parasitism.