ATTACHMENT OF SMOOTH-MUSCLE CELLS TO COLLAGEN AND THEIR MIGRATION TOWARD PLATELET-DERIVED GROWTH-FACTOR
ATTACHMENT OF SMOOTH-MUSCLE CELLS TO COLLAGEN AND THEIR MIGRATION TOWARD PLATELET-DERIVED GROWTH-FACTOR
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DOI：
10.1073/pnas.78.6.3669
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
MARTIN, GR
中科院分区：
文献类型：
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作者：
GROTENDORST, GR;SEPPA, HEJ;MARTIN, GR
来自胎羊的平滑肌细胞使用纤连蛋白结合I型和III型胶原，但通过胰蛋白酶抗性内在糖缀合物结合V型胶原。V型胶原蛋白上的结合位点位于α A链中。通过在改良的Boyden室趋化性试验中使用胶原蛋白包被的过滤器，观察到血小板衍生生长因子对平滑肌细胞具有趋化性，但其他几种生长因子无活性。平滑肌细胞从血管中膜向内膜的迁移导致动脉粥样硬化斑块的形成，这可能是响应血小板衍生生长因子的细胞趋化性迁移的结果。
Smooth muscle cells from fetal sheep use fibronectin to bind to type I and type III collagens but bind to type V collagen by a trypsin-resistant intrinsic glycoconjugate. The binding site on type V collagen is located in the .alpha.A chain. By using collagen-coated filters in a modified Boyden chamber assay for chemotaxis, it was observed that the platelet-derived growth factor was chemotactic for smooth muscle cells but that several other growth factors were inactive. The migration of smooth muscle cells from the media to the intima of a blood vessel, which leads to the formation of an atherosclerotic plaque, may be the result of a chemotactic migration of cells responsive to the platelet-derived growth factor.