Hypoxia and extracellular vesicles: A review on methods, vesicular cargo and functions.
Hypoxia and extracellular vesicles: A review on methods, vesicular cargo and functions.
复制标题
缺氧和细胞外囊泡：有关方法，囊泡货物和功能的综述。
DOI：
10.1002/jev2.12002
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发表时间：
2020-10
影响因子：
16
通讯作者：
Malm T
中科院分区：
文献类型：
--
作者：
Bister N;Pistono C;Huremagic B;Jolkkonen J;Giugno R;Malm T
缺氧是几种严重疾病的基本标志，如心血管和代谢紊乱以及癌症。组织氧水平的下降诱导细胞的缺氧反应，这些细胞努力适应变化的条件。不能适应长时间或严重的缺氧会引发细胞死亡。虽然一些细胞类型，如神经元，对缺氧非常敏感，但癌细胞利用缺氧环境进行肿瘤生长，血管生成和转移。缺氧诱导的过程触发复杂的细胞间通讯，现在有迹象表明细胞外囊泡（EV）在这些过程中发挥着重要作用。EV分离和表征方法的最新发展提高了对EV纯度在功能和货物研究中的重要性的认识。细胞死亡是严重缺氧的标志，是孤立EV中细胞内污染物的已知来源。在这篇综述中，对研究缺氧诱导EV的方法学方面进行了严格评价。重点关注和差距，目前的知识和未来的研究方向进行了设置。为了加速和推进研究，与常氧EV相比，对低氧EV的功能和货物进行了深入分析，重点是EV的microRNA含量改变。
Hypoxia is an essential hallmark of several serious diseases such as cardiovascular and metabolic disorders and cancer. A decline in the tissue oxygen level induces hypoxic responses in cells which strive to adapt to the changed conditions. A failure to adapt to prolonged or severe hypoxia can trigger cell death. While some cell types, such as neurons, are highly vulnerable to hypoxia, cancer cells take advantage of a hypoxic environment to undergo tumour growth, angiogenesis and metastasis. Hypoxia‐induced processes trigger complex intercellular communication and there are now indications that extracellular vesicles (EVs) play a fundamental role in these processes. Recent developments in EV isolation and characterization methodology have increased the awareness of the importance of EV purity in functional and cargo studies. Cell death, a hallmark of severe hypoxia, is a known source of intracellular contaminants in isolated EVs. In this review, methodological aspects of studies investigating hypoxia‐induced EVs are critically evaluated. Key concerns and gaps in the current knowledge are highlighted and future directions for studies are set. To accelerate and advance research, an in‐depth analysis of the functions and cargo of hypoxic EVs, compared to normoxic EVs, is provided with the focus on the altered microRNA contents of the EVs.
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影响因子：
3.7
作者：
Feng Y;Huang W;Wani M;Yu X;Ashraf M
通讯作者：
Ashraf M
DOI：
10.33594/000000102
发表时间：
2019-01-01
期刊：
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
影响因子：
--
作者：
Collino, Federica;Lopes, Jarlene Alecia;Lindoso, Rafael Soares
通讯作者：
Lindoso, Rafael Soares
影响因子：
7.2
作者：
Berchem G;Noman MZ;Bosseler M;Paggetti J;Baconnais S;Le Cam E;Nanbakhsh A;Moussay E;Mami-Chouaib F;Janji B;Chouaib S
通讯作者：
Chouaib S
影响因子：
4.6
作者：
Benedikter BJ;Bouwman FG;Vajen T;Heinzmann ACA;Grauls G;Mariman EC;Wouters EFM;Savelkoul PH;Lopez-Iglesias C;Koenen RR;Rohde GGU;Stassen FRM
通讯作者：
Stassen FRM
影响因子：
5.3
作者：
de Jong OG;van Balkom BW;Gremmels H;Verhaar MC
通讯作者：
Verhaar MC