BREAST TUMOR-CELL LINES FROM PLEURAL EFFUSIONS
BREAST TUMOR-CELL LINES FROM PLEURAL EFFUSIONS
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DOI：
10.1093/jnci/53.3.661
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发表时间：
1974-01-01
期刊：
影响因子：
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通讯作者：
REEVES, WJ
中科院分区：
文献类型：
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作者：
CAILLEAU, R;YOUNG, R;REEVES, WJ
1973年，从乳腺癌患者的胸腔积液中分离出4种新的上皮肿瘤细胞系。我们描述了其中3个品系：MDA-MB-134，平均染色体数为43; MDA-MB-175，平均染色体数为49; MDA-MB-231，平均染色体数在65和69之间。我们从MDA-MB-134的10例渗出液中的4例和MDA-MB-175的8例渗出液中的6例中分离出相同的细胞类型。我们发现胸腔积液作为体外培养和研究乳腺肿瘤细胞的来源具有以下优点：1）大量的材料和从同一患者获得连续样品的可能性; 2）肿瘤细胞的高活力; 3）成纤维细胞的稀缺或缺乏;和4）通过肿瘤细胞与培养瓶的附着速度或程度的差异将肿瘤细胞与其它“污染”细胞类型分离的可能性。从肉眼和显微镜下观察，来自不同患者的所有线均不同。来自同一患者的连续胸腔积液的所有线明显相似。在所有生产线中均未检出病毒或支原体。
During 1973, 4 new epithelial tumor cell lines were isolated from pleural effusions from breast cancer patients. We describe 3 of these lines: MDA-MB-134, with a mean chromosome number of 43; MDA-MB-175, with a mean chromosome number of 49; and MDA-MB-231, with a mean chromosome number between 65 and 69. We isolated the same cell type from 4 of 10 effusions from MDA-MB-134 and from 6 of 8 effusions from MDA-MB-175. We found that pleural effusions as a source of breast tumor cells to be cultured and studied in vitro have the following advantages: 1) large amounts of material and the possibility of obtaining sequential samples from the same patient; 2) high viability of tumor cells; 3) scarcity or absence of fibroblasts; and 4) the possibility of separating the tumor cells from other “contaminating” cell types by differences in their speed or degree of attachment to the flask. All lines from different patients differed, as seen grossly and microscopically. All lines from sequential pleural effusions from the same patient were apparently alike. No viruses or mycoplasmas were detected in any line.