Bacterial residence time in the intestine of Caenorhabditis elegans
Bacterial residence time in the intestine of Caenorhabditis elegans
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DOI：
10.1163/156854107779969718
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发表时间：
2007-01-01
期刊：
影响因子：
1.2
通讯作者：
McGhee, James D.
中科院分区：
文献类型：
--
作者：
Ghafouri, Shervin;McGhee, James D.
我们将成年秀丽隐杆线虫的荧光微球与其大肠杆菌食物混合，然后在转移到未标记的细菌后，测量排出的粪便的总荧光随时间的变化。肠道清除率遵循简单的一阶衰减或稀释曲线：我们估计每次排便排出最大肠道体积的43 +/- 10%，并且头部（由此推断，细菌）的平均停留时间小于2分钟。我们的研究结果提出了一个问题，即细菌如何在这么短的时间内被充分消化，以提供能量和物质来维持秀丽隐杆线虫卵母细胞的高产率。
We fed adult Caenorhabditis elegans fluorescent microspheres mixed with their Escherichia coli food and then measured the total fluorescence of expelled faeces as a function of time after transfer to unlabelled bacteria. Intestinal clearance obeys a simple first-order decay or dilution curve: we estimate that 43 +/- 10% of the maximum intestinal volume is expelled in each defecation and the average residence time of a bead (by inference, a bacterium) is less than 2 min. Our results raise questions how bacteria can be sufficiently digested in this brief period to provide energy and material to sustain the high rate of C. elegans oocyte production.