STRUCTURAL FEATURES OF ISOLATED GENERATIVE CELLS AND THEIR PROTOPLASTS FROM POLLEN OF SOME LILIACEOUS PLANTS
STRUCTURAL FEATURES OF ISOLATED GENERATIVE CELLS AND THEIR PROTOPLASTS FROM POLLEN OF SOME LILIACEOUS PLANTS
复制标题
DOI：
10.1002/mrd.1120240403
复制
发表时间：
1989-12-01
期刊：
影响因子：
--
通讯作者：
MIKIHIROSIGE, H
中科院分区：
文献类型：
--
作者：
TANAKA, I;NAKAMURA, S;MIKIHIROSIGE, H
Large quantities of intact generative cells and their protoplasts were isolated from pollen protoplasts of four lilaceous plants, and their structural features were investigated. The generative cells, liberated from the vegetative cell cytoplasm of the pollen protoplasts, were initially spindle-shaped with two long, oppositely orientated extensions, and were surrounded by two cell membranes one on each side of a wall of uniform thickness. The generative nuclei, stained with 4''-6-diamidino-2-phenylindole (DAPI), showed ellipsoidal and highly condensed chromatin, whereas the generative cell cytoplasm, whose quantity was widely different from species to species showed no fluorescence, suggesting the absence of plastid and mitochondrial DNA, although many mitochondria were present. The isolated generative cells, which were spindle-shaped at first, became spherical in shape in vitro. Immunocytochemistry and transmission electron microscopy revealed that this change was associated with the depolymerization of an axial array of microtubules present in generative cells in situ. These results are discussed in relation to the function of the generative cell within the bicellular pollen of angiosperms.
