STUDIES ON HISTONE ORGANIZATION IN NUCLEOSOME USING FORMALDEHYDE AS A REVERSIBLE CROSS-LINKING AGENT
STUDIES ON HISTONE ORGANIZATION IN NUCLEOSOME USING FORMALDEHYDE AS A REVERSIBLE CROSS-LINKING AGENT
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DOI：
10.1016/0092-8674(78)90278-7
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发表时间：
1978-01-01
期刊：
影响因子：
64.5
通讯作者：
JACKSON, V
中科院分区：
文献类型：
--
作者：
JACKSON, V
描述了一种新的程序，允许选择性逆转甲醛交联在两个组蛋白组蛋白和组蛋白dna的细胞核分离小牛胸腺。观察到4种非h1组蛋白H3、H2B、H2A和H4的10种可能二聚体，主要二聚体为H3-H3、H3-H2A、H2B-H2A、H2A -H2A和H2B-H4的2种单独二聚体。虽然非h1组蛋白的低聚物是通过这种试剂的长期处理形成的，但50%的组蛋白仍然对彼此交联具有抗性。对于那些交联的组蛋白，分子内的交联位点位于核心（胰蛋白酶抗性）区域，因此表明这些分子在核小体中的接近性。核心区域还与DNA交联，表明该区域在所有非h1组蛋白与DNA之间存在密切的相互作用。
A new procedure is described which allows selective reversal of formaldehyde cross-linking in both histone-histone and histone-DNA of nuclei isolated from calf thymus. All 10 possible dimers of the 4 non-H1 histones, H3, H2B, H2A and H4, are observed, the major dimers being H3-H3, H3-H2A, H2B-H2A, H2a-H2A and 2 separate dimers of H2B-H4. Although oligomers of the non-H1 histones are formed by prolonged treatment with this reagent, 50% of the histones continue to remain resistant to cross-linking with each other. For those histones which cross-link, the site of cross-linking within the molecules is located in the core (trypsin-resistant) region and therefore indicates proximities for these molecules in the nucleosome. The core region also cross-links to DNA, indicating intimate interactions between this region in all the non-H1 histones with DNA.