A basic helix-loop-helix transcription factor DvIVS determines flower color intensity in cyanic dahlia cultivars
A basic helix-loop-helix transcription factor DvIVS determines flower color intensity in cyanic dahlia cultivars
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DOI：
10.1007/s00425-013-1897-x
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发表时间：
2013-08-01
期刊：
影响因子：
4.3
通讯作者：
Doi, Motoaki
中科院分区：
文献类型：
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作者：
Ohno, Sho;Deguchi, Ayumi;Doi, Motoaki
该研究旨在利用 15 个花瓣颜色强度不同的品种，确定调节蓝绿色大丽花 (Dahlia variabilis) 花朵颜色强度的因素。根据其类黄酮成分，这些品种被分为三类：象牙白类黄酮类品种；含有黄酮和花青素的紫色和粉红色品种；以及含有黄酮、花青素和查耳酮的红色品种。在紫色、粉色和象牙白色品种中，亮度之间存在反比关系，亮度被用作颜色强度和花青素含量的指标。花青素含量与花青素合成途径中受基本螺旋-环-螺旋转录因子DvIVS调控的一些结构基因的表达呈正相关。还发现花青素含量与 DvIVS 表达呈正相关。 DvIVS的启动子区域分为三种类型，携带1型启动子的品种颜色较深，携带2型和/或3型启动子的品种颜色较浅，携带1型和2型和/或3型启动子的品种颜色中等。来自这些启动子的基因转录物编码全长预测蛋白质。这些结果表明DvIVS启动子区域的基因型是决定花色强度的关键因素之一。
The study was aimed to identify the factors that regulate the intensity of flower color in cyanic dahlia (Dahlia variabilis), using fifteen cultivars with different color intensities in their petals. The cultivars were classified into three groups based on their flavonoid composition: ivory white cultivars with flavones; purple and pink cultivars with flavones and anthocyanins; and red cultivars with flavones, anthocyanins, and chalcones. Among the purple, pink, and ivory white cultivars, an inverse relationship was detected between lightness, which was used as an indicator for color intensity and anthocyanin content. A positive correlation was detected between anthocyanin contents and the expression of some structural genes in the anthocyanin synthesis pathway that are regulated by DvIVS, a basic helix-loop-helix transcription factor. A positive correlation between anthocyanin content and expression of DvIVS was also found. The promoter region of DvIVS was classified into three types, with cultivars carrying Type 1 promoter exhibited deep coloring, those carrying Type 2 and/or Type 3 exhibited pale coloring, and those carrying Type 1 and Type 2 and/or Type 3 exhibited medium coloring. The transcripts of the genes from these promoters encoded full-length predicted proteins. These results suggested that the genotype of the promoter region in DvIVS is one of the key factors determining the flower color intensity.