Nogo-66 Receptor at Cerebellar Cortical Glia Gap Junctions in the Rat
Nogo-66 Receptor at Cerebellar Cortical Glia Gap Junctions in the Rat
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DOI：
10.1159/000086291
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发表时间：
2005-07
期刊：
影响因子：
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通讯作者：
Xia Liu;Yingying Liu;Wei-lin Jin;Huiling Liu;G. Ju
中科院分区：
文献类型：
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作者：
Xia Liu;Yingying Liu;Wei-lin Jin;Huiling Liu;G. Ju
Nogo-A是神经突生长的髓磷脂抑制剂，是中枢神经系统纤维再生困难的原因。它的66个氨基酸胞外结构域（Nogo-66）有助于抑制Nogo-A的活性。Nogo-66受体广泛分布于中枢神经系统的神经元中，包括小脑。在我们对大鼠小脑皮层Nogo-66受体分布的研究中，我们意外地发现Nogo-66受体在神经胶质细胞中具有免疫反应性，特别是在浦肯野细胞下方。Nogo-66受体在神经胶质细胞中的存在尚未见报道。因此进行了详细的研究。免疫电镜研究清楚地表明，Nogo-66受体的免疫反应性可以在胶质细胞间的间隙连接处确定，表明Nogo-66受体可能通过间隙连接调节胶质细胞间的通讯。
Nogo-A is a myelin inhibitor of neurite outgrowth that accounts for the difficulty in fiber regeneration in the central nervous system. Its 66-amino-acid extracellular domain (Nogo-66) contributes to the inhibitory activity of Nogo-A. The Nogo-66 receptor is widely distributed in neurons of the central nervous system, including the cerebellum. In our study on the distribution of Nogo-66 receptor in the cerebellar cortex in the rat, we unexpectedly found Nogo-66 receptor immunoreactivity in the glia cells, particularly abundant beneath the Purkinje cells. The presence of Nogo-66 receptor in glia cells has not been reported before. A detailed study was thus conducted. Immunoelectron microscopic investigation clearly demonstrated that the Nogo-66 receptor immunoreactivity could be ascertained at the gap junction between glia cells, indicating that the Nogo-66 receptor may modulate the communication between glia cells through gap junctions.