Rat aversion to sevoflurane and isoflurane
Rat aversion to sevoflurane and isoflurane
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DOI：
10.1016/j.applanim.2014.12.013
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发表时间：
2015-03-01
影响因子：
2.3
通讯作者：
Weary, Daniel M.
中科院分区：
文献类型：
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作者：
Bertolus, Julie Boulanger;Nemeth, Geoff;Weary, Daniel M.
几乎所有用于研究的啮齿动物最终都被安乐死。最佳做法是在安乐死前使用卤化麻醉剂（如异氟烷）麻醉这些动物。暴露于异氟烷是令人厌恶的，但不如暴露于常用的二氧化碳。七氟烷是一种较新的卤代麻醉剂，可在人体中提供更平稳的诱导，但尚未研究啮齿动物对增加七氟烷浓度的厌恶。本研究的目的是使用厌恶-回避和接近-回避测试比较大鼠对异氟烷和七氟烷的厌恶。在前者中，大鼠在明/暗箱中进行测试，并可以选择留在充满麻醉剂的首选暗室中，或逃到令人厌恶的明亮室中。在后者中，大鼠可以选择在充满麻醉剂的房间中获得甜食奖励，或者逃离房间并放弃奖励。在回避-回避实验中，两种麻醉剂之间在保持在暗室中直至横卧的大鼠数量方面没有差异（异氟烷和七氟烷暴露分别为11/17和12/17）;然而，第二次暴露期间大鼠耐受麻醉剂暴露的可能性仅为第一次暴露期间的一半（P=0.049）。在接近-回避实验中，两种麻醉剂在放弃食物奖励以逃避暴露的大鼠数量上没有差异。在初始暴露期间，18只大鼠中有7只在下室中躺卧，而在再次暴露期间，16只大鼠中仅有1只（P=0.001）。我们得出结论，大鼠发现异氟烷和七氟烷同样令人厌恶，这两种药物在再次暴露后变得更加令人厌恶。(C)2015 Elsevier B. V.版权所有。
Virtually all rodents used in research are eventually euthanized. Best practice is to anaesthetize these animals before euthanasia using a halogenated anaesthetic such as isoflurane. Exposure to isoflurane is aversive, but less so than exposure to the commonly used carbon dioxide. Sevoflurane is a newer halogenated anaesthetic that provides a smoother induction in humans, but rodent aversion to increasing concentrations of sevoflurane has yet to be investigated. The aim of this study was to compare rat aversion to isoflurane and sevoflurane using aversion-avoidance and approach-avoidance testing. In the former, rats were tested in a light/dark box and had the choice between remaining in a preferred dark chamber filling with an anaesthetic, or escaping to an aversive bright chamber. In the latter, rats had the choice between accessing a sweet food reward in a chamber filling with an anaesthetic or escaping the chamber and forgoing the reward. In the aversion-avoidance experiment, there was no difference between the two anaesthetics in the number of rats that remained in the dark chamber until they became recumbent (11 and 12 out of 17 for isoflurane and sevoflurane exposure, respectively); however, rats were only half as likely to tolerate exposure to the anaesthetics during the second exposure than during the first (P=0.049). In the approach-avoidance experiment, there was no difference between the two anaesthetics in the number of rats that abandoned the food reward to escape exposure. During the initial exposure, 7 of 18 rats became recumbent in the lower chamber versus just 1 of 16 rats during re-exposure (P=0.001). We conclude that rats find isoflurane and sevoflurane similarly aversive and that both agents become more aversive upon re-exposure. (C) 2015 Elsevier B.V. All rights reserved.