Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae
Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae
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DOI：
10.1093/nar/24.22.4420
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发表时间：
1996-11-15
影响因子：
14.9
通讯作者：
Herrmann, R
中科院分区：
文献类型：
--
作者：
Himmelreich, R;Hilbert, H;Herrmann, R
测定了肺炎支原体M129的全基因组序列，其大小为816394 bp，平均G + C含量为40.0mol%。预测了677个开放阅读框（ORF）和39个编码各种RNA的基因。75.9%与其他生物的基因/蛋白质有显著相似性，只有9.9%与数据库中的基因序列没有显著相似性，这使我们能够尝试性地对大量的ORF进行功能分类，并推断该细菌的生化和生理特性。肺炎克雷伯氏菌在其从祖先细菌的还原进化过程中的死亡可以通过完全合成代谢（例如没有氨基酸合成）和代谢途径的丧失来解释。肺炎在自然界中依赖于专性寄生的生活方式！这需要提供外源性必需代谢物，所有主要类别的细胞过程和代谢途径进行了简要描述，对于一些活动/功能存在于M.根据实验证据，通过相似性搜索不能鉴定相应的基因，例如，我们未能鉴定参与运动性、趋化性和氧化应激管理的基因/蛋白。
The entire genome of the bacterium Mycoplasma pneumoniae M129 has been sequenced, It has a size of 816 394 base pairs with an average G + C content of 40.0 mol%, We predict 677 open reading frames (ORFs) and 39 genes coding for various RNA species, Of the predicted ORFs, 75.9% showed significant similarity to genes/proteins of other organisms while only 9.9% did not reveal any significant similarity to gene sequences in databases, This permitted us tentatively to assign a functional classification to a large number of ORFs and to deduce the biochemical and physiological properties of this bacterium, The reduction of the genome size of M. pneumoniae during its reductive evolution from ancestral bacteria can be explained by the loss of complete anabolic (e.g. no amino acid synthesis) and metabolic pathways, Therefore, M. pneumoniae depends in nature on an obligate parasitic lifestyle! which requires the provision of exogenous essential metabolites, All the major classes of cellular processes and metabolic pathways are briefly described, For a number of activities/functions present in M. pneumoniae according to experimental evidence, the corresponding genes could not be identified by similarity search, For instance we failed to identify genes/proteins involved in motility, chemotaxis and management of oxidative stress.