INVIVO MICRODIALYSIS MEASUREMENT OF HISTAMINE IN RAT-BLOOD EFFECTS OF COMPOUND-48/80 AND HISTAMINE-RECEPTOR ANTAGONISTS
INVIVO MICRODIALYSIS MEASUREMENT OF HISTAMINE IN RAT-BLOOD EFFECTS OF COMPOUND-48/80 AND HISTAMINE-RECEPTOR ANTAGONISTS
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DOI：
10.1016/1056-8719(93)90058-m
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发表时间：
1993-04-01
影响因子：
1.9
通讯作者：
WATANABE, T
中科院分区：
文献类型：
--
作者：
SAKURAI, E;GUNJI, E;WATANABE, T
一种在体微透析方法结合一个高灵敏度的HPLC方法，这是开发用于分析的介质在中枢神经系统已被应用于测定组胺在血液中的浓度。本文应用该技术研究了化合物48/80和组胺受体拮抗剂对大鼠血中组胺释放的影响。通过体内微透析测量的血液中组胺的平均基础水平为177.8 +/- 11.1 pmol/mL。该水平不受腹膜内（i. p.）注射生理盐水，并在注射生理盐水后保持恒定水平至少8小时。腹腔注射组胺（0.5 mg/kg）后，在颈静脉血液中迅速检测到组胺。此外，由于透析液中回收的组胺与血液中的游离部分成正比，因此血液的体内微透析方法是检查组胺释放到血液中的可靠方法。在我们的实验中，化合物48/80（2.0 mg/kg，i. p.）显著增加大鼠颈静脉透析液中的组胺水平，表明组胺从肥大细胞释放到血液中。然而，在腹膜内注射组胺受体拮抗剂如吡拉明（2.0 mg/kg）、d-氯苯那敏（2.0 mg/kg）、西咪替丁（10 mg/kg）或硫代哌丁胺（10 mg/kg）后，组胺浓度没有增加。因此，目前的结果表明，这些组胺受体拮抗剂可能不会对组胺释放到血液中的影响。
An in vivo microdialysis method combined with a highly sensitive HPLC method which was developed for the analysis of the mediators in the CNS has been applied to assay histamine concentrations in the blood. The technique was used to study the effects of compound 48/80 and histamine receptor antagonists on histamine release in the blood of rats. The mean basal level of histamine in the blood measured by in vivo microdialysis was 177.8 +/- 11.1 pmol/mL. This level was not affected significantly by intraperitoneal (i.p.) injection of saline, and remained at the constant level for at least 8 hr after injection of saline. After i.p. injection of histamine (0.5 mg/kg), histamine was quickly detected in the blood of the jugular vein. Moreover, because the recovered histamine in the dialysate is directly proportional to the free fraction in the blood, the in vivo microdialysis method of blood is a reliable method of examining histamine release into the blood. In our experiments, the histamine level in dialysates from rat jugular vein was markedly increased by compound 48/80 (2.0 mg/kg, i.p.), demonstrating the histamine release into the blood from mast cells. However, there was no increase in histamine concentration after an i.p. injection of histamine receptor antagonists, such as pyrilamine (2.0 mg/kg), d-chlorpheniramine (2.0 mg/kg), cimetidine (10 mg/kg), or thioperamide (10 mg/kg). Thus, the present results suggested that these histamine receptor antagonists might not have an influence on histamine release into the blood.