EFFECT OF TISSUE PROCESSING ON COLLOIDAL GOLD CYTOCHEMISTRY
EFFECT OF TISSUE PROCESSING ON COLLOIDAL GOLD CYTOCHEMISTRY
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DOI：
10.1177/35.9.3302022
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发表时间：
1987-09-01
影响因子：
3.2
通讯作者：
KAN, FWK
中科院分区：
文献类型：
--
作者：
BENDAYAN, M;NANCI, A;KAN, FWK
细胞化学技术的目的是在特定的组织和细胞隔间定位特定的生化成分。然而，由于组织结构观察的准备会导致其组成成分的性质发生重大变化，因此一个主要问题是如何保持足够程度的生化性质以及充分的结构保存。在目前的研究中，我们描述了使用不同的胶体金细胞化学技术对通过不同方法处理的组织所获得的结果。我们发现，在加工过程中对组织的任何操作都可能导致组织成分的修改，从而导致细胞化学问题。事实上，固定前组织的清洗、固定液的性质、树脂的化学基础以及包埋的物理条件都会导致组织成分的变化，这些变化可以通过细胞化学来证明。蛋白A-金、凝集素-金和酶-金细胞化学技术在提交给不同处理的组织上的应用说明了这一点：通过灌流或浸泡固定；戊二醛与多聚甲醛固定液；冷冻-超微切割法；在环氧树脂、GMA、Lowicryl或LR树脂中包埋。所获得的结果表明，必须为每一类结合位点确定最佳标记条件，并且在细胞化学中不能推荐任何单一的方法作为最佳方法。
The aim of cytochemical techniques is to localize specific biochemical components in particular tissue and cell compartments. However, since preparation of tissues for structural observation results in major alterations of the properties of their components, a major problem is to retain an adequate degree of their biochemical properties as well as adequate structural preservation. In the present study, we describe results obtained using various colloidal gold cytochemical techniques on tissues processed through different approaches. We found that any manipulation of the tissue during its processing can result in modifications of tissue components, leading to problems in cytochemistry. Indeed, washing of the tissue before fixation, the nature of the fixative solution, the chemical basis of the resins, and the physical conditions of embedding can all introduce changes in tissue components which can be cytochemically demonstrated. This has been illustrated with application of the protein A-gold, lectin-gold, and enzyme-gold cytochemical techniques on tissues submitted to different processings: fixation by perfusion or by immersion; glutaraldehyde vs paraformaldehyde fixative solutions; cryo-ultramicrotomy; embedding in epoxy, GMA, Lowicryl, or LR resins. The results obtained have demonstrated that conditions for optimal labeling must be worked out for each class of binding sites, and that no single procedure can be recommended as THE best approach in cytochemistry.